Document Detail


Identification and functional characterization of the Varicella zoster virus ORF11 gene product.
MedLine Citation:
PMID:  21276599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The deletion of ORF11 severely impaired VZV infection of human skin xenografts. Here, we investigate the characteristics and functions of the ORF11 gene product. ORF11 is expressed as a 118kDa polypeptide in VZV-infected cells; the protein is present in the nucleus and cytoplasm and is incorporated into VZ virions. Although ORF11 had little effect in transactivating VZV gene promoters in transfection assays, deleting ORF11 from the virus was associated with reduced expression of immediate early proteins IE4, IE62 and IE63, and the major glycoprotein, gE. ORF11 was identified as an RNA binding protein and its RNA binding domain was defined. However, disrupting the ORF11 RNA binding domain did not affect skin infection, indicating that RNA binding capacity, conserved among the alphaherpesviruses homologues, is not essential while the contribution of ORF11 to the expression of the IE proteins and gE may be required for VZV pathogenesis in skin in vivo.
Authors:
Xibing Che; Stefan L Oliver; Marvin H Sommer; Jaya Rajamani; Mike Reichelt; Ann M Arvin
Related Documents :
21531719 - Deimination of human filaggrin-2 promotes its proteolysis by calpain 1.
21331069 - The self-association coiled-coil domain of pml is sufficient for the oncogenic conversi...
19828839 - Developmental and functional studies of the slc12 gene family members from drosophila m...
356049 - Identification of the structural proteins of an atp-driven potassium transport system i...
964259 - Identification of a membrane protein involved in mitochondrial phosphate transport.
1549599 - Mechanism of maltose transport in escherichia coli: transmembrane signaling by periplas...
8405379 - Mos is degraded by the 26s proteasome in a ubiquitin-dependent fashion.
8939939 - A novel abetalipoproteinemia genotype. identification of a missense mutation in the 97-...
8288629 - A novel cytokine exhibiting megakaryocyte potentiating activity from a human pancreatic...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-26
Journal Detail:
Title:  Virology     Volume:  412     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-14     Completed Date:  2011-05-03     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  156-66     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / chemistry
Cytoplasm / chemistry
Gene Deletion
Gene Expression
Gene Expression Profiling
Gene Expression Regulation, Viral
Genes, Viral
Herpesvirus 3, Human / genetics*,  pathogenicity*
Humans
Immediate-Early Proteins / biosynthesis
Molecular Weight
Open Reading Frames*
RNA-Binding Proteins / chemistry,  genetics,  physiology*
Skin / pathology,  virology
Transplantation, Heterologous
Viral Structural Proteins / chemistry,  genetics,  physiology*
Virulence Factors / chemistry,  genetics,  physiology*
Grant Support
ID/Acronym/Agency:
AI053846/AI/NIAID NIH HHS; AI20459/AI/NIAID NIH HHS; R01 AI053846/AI/NIAID NIH HHS; R01 AI053846-08/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Immediate-Early Proteins; 0/RNA-Binding Proteins; 0/Viral Structural Proteins; 0/Virulence Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Disappearance of some human African trypanosomiasis transmission foci in Zambia in the absence of a ...
Next Document:  Regulation of human papillomavirus type 16 early gene expression in trophoblastic and cervical cells...