| Identification and functional characterization of the Varicella zoster virus ORF11 gene product. | |
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MedLine Citation:
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PMID: 21276599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The deletion of ORF11 severely impaired VZV infection of human skin xenografts. Here, we investigate the characteristics and functions of the ORF11 gene product. ORF11 is expressed as a 118kDa polypeptide in VZV-infected cells; the protein is present in the nucleus and cytoplasm and is incorporated into VZ virions. Although ORF11 had little effect in transactivating VZV gene promoters in transfection assays, deleting ORF11 from the virus was associated with reduced expression of immediate early proteins IE4, IE62 and IE63, and the major glycoprotein, gE. ORF11 was identified as an RNA binding protein and its RNA binding domain was defined. However, disrupting the ORF11 RNA binding domain did not affect skin infection, indicating that RNA binding capacity, conserved among the alphaherpesviruses homologues, is not essential while the contribution of ORF11 to the expression of the IE proteins and gE may be required for VZV pathogenesis in skin in vivo. |
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Authors:
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Xibing Che; Stefan L Oliver; Marvin H Sommer; Jaya Rajamani; Mike Reichelt; Ann M Arvin |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-01-26 |
Journal Detail:
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Title: Virology Volume: 412 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-14 Completed Date: 2011-05-03 Revised Date: 2012-09-20 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 156-66 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Departments of Pediatrics and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. xibing@stanford.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Nucleus
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chemistry Cytoplasm / chemistry Gene Deletion Gene Expression Gene Expression Profiling Gene Expression Regulation, Viral Genes, Viral Herpesvirus 3, Human / genetics*, pathogenicity* Humans Immediate-Early Proteins / biosynthesis Molecular Weight Open Reading Frames* RNA-Binding Proteins / chemistry, genetics, physiology* Skin / pathology, virology Transplantation, Heterologous Viral Structural Proteins / chemistry, genetics, physiology* Virulence Factors / chemistry, genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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AI053846/AI/NIAID NIH HHS; AI20459/AI/NIAID NIH HHS; R01 AI053846-08/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immediate-Early Proteins; 0/RNA-Binding Proteins; 0/Viral Structural Proteins; 0/Virulence Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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