Document Detail

Identification and functional characterization of the Varicella zoster virus ORF11 gene product.
MedLine Citation:
PMID:  21276599     Owner:  NLM     Status:  MEDLINE    
The deletion of ORF11 severely impaired VZV infection of human skin xenografts. Here, we investigate the characteristics and functions of the ORF11 gene product. ORF11 is expressed as a 118kDa polypeptide in VZV-infected cells; the protein is present in the nucleus and cytoplasm and is incorporated into VZ virions. Although ORF11 had little effect in transactivating VZV gene promoters in transfection assays, deleting ORF11 from the virus was associated with reduced expression of immediate early proteins IE4, IE62 and IE63, and the major glycoprotein, gE. ORF11 was identified as an RNA binding protein and its RNA binding domain was defined. However, disrupting the ORF11 RNA binding domain did not affect skin infection, indicating that RNA binding capacity, conserved among the alphaherpesviruses homologues, is not essential while the contribution of ORF11 to the expression of the IE proteins and gE may be required for VZV pathogenesis in skin in vivo.
Xibing Che; Stefan L Oliver; Marvin H Sommer; Jaya Rajamani; Mike Reichelt; Ann M Arvin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-26
Journal Detail:
Title:  Virology     Volume:  412     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-14     Completed Date:  2011-05-03     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  156-66     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Cell Nucleus / chemistry
Cytoplasm / chemistry
Gene Deletion
Gene Expression
Gene Expression Profiling
Gene Expression Regulation, Viral
Genes, Viral
Herpesvirus 3, Human / genetics*,  pathogenicity*
Immediate-Early Proteins / biosynthesis
Molecular Weight
Open Reading Frames*
RNA-Binding Proteins / chemistry,  genetics,  physiology*
Skin / pathology,  virology
Transplantation, Heterologous
Viral Structural Proteins / chemistry,  genetics,  physiology*
Virulence Factors / chemistry,  genetics,  physiology*
Grant Support
Reg. No./Substance:
0/Immediate-Early Proteins; 0/RNA-Binding Proteins; 0/Viral Structural Proteins; 0/Virulence Factors

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