Document Detail


Identification and functional characterization of the Lactococcus lactis CodY-regulated branched-chain amino acid permease BcaP (CtrA).
MedLine Citation:
PMID:  16621821     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcriptome analyses have previously revealed that a gene encoding the putative amino acid transporter CtrA (YhdG) is one of the major targets of the pleiotropic regulator CodY in Lactococcus lactis and Bacillus subtilis. The role of ctrA in L. lactis was further investigated with respect to both transport activity as well as CodY-mediated regulation. CtrA is required for optimal growth in media containing free amino acids as the only amino acid source. Amino acid transport studies showed that ctrA encodes a secondary amino acid transport system that is specific for branched-chain amino acids (BCAAs) (isoleucine, leucine, and valine) and methionine, which is in disagreement with its previously proposed function (a cationic amino acid transporter), which was assigned based on homology. We propose to rename CtrA BcaP, for branched-chain amino acid permease. BcaP is a member of a group of conserved transport systems, as homologs are widely distributed among gram-positive bacteria. Deletion of bcaP resulted in the loss of most of the BCAA uptake activity of L. lactis, indicating that BcaP is the major BCAA carrier of this organism. Deletion of bcaP together with a second (putative) BCAA permease, encoded by brnQ, further reduced the viability of the strain. DNA microarray analysis showed that deletion of bcaP predominantly affects genes belonging to the regulons of the transcriptional regulator CodY, which is involved in global nitrogen metabolism and needs BCAAs for its activation, and of CmbR, which is involved in sulfur amino acid metabolism.
Authors:
Chris D den Hengst; Maarten Groeneveld; Oscar P Kuipers; Jan Kok
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  188     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-19     Completed Date:  2006-06-12     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3280-9     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ377686;  DQ377687
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Transport Systems / genetics,  metabolism*
Amino Acids, Branched-Chain / metabolism*
Amino Acids, Sulfur / metabolism
Bacterial Proteins / genetics,  metabolism*
DNA-Binding Proteins / genetics,  metabolism
Gene Expression Regulation, Bacterial
Genes, Bacterial
Lactococcus lactis / enzymology*,  genetics,  growth & development
Molecular Sequence Data
Nitrogen / metabolism
Repressor Proteins / genetics,  metabolism
Transcription Factors / metabolism
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/Amino Acids, Branched-Chain; 0/Amino Acids, Sulfur; 0/Bacterial Proteins; 0/CmbR protein, Lactococcus lactis; 0/CtrA protein, Caulobacter; 0/DNA-Binding Proteins; 0/Repressor Proteins; 0/Transcription Factors; 7727-37-9/Nitrogen
Comments/Corrections

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