Document Detail


Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish.
MedLine Citation:
PMID:  21268182     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies suggest that egg activation in mammals is triggered by a sperm-specific phospholipase C, PLCzeta. In other vertebrate species such as medaka fish, chickens, and quail, PLCzeta is also expressed as a testis-specific mRNA. Functional studies suggest that PLCzeta plays a similar role as a trigger of egg activation in these species. Here, we report the identification of PLCzeta orthologues in pufferfish species Takifugu rubripes (Fugu) and Tetraodon nigroviridis (Tetraodon). Unexpectedly in these species PLCzeta is expressed not in the testis, but in ovary and brain. Injection of pufferfish PLCzeta copy ribonucleic acid (cRNA) into mouse eggs failed to trigger calcium oscillations, unlike medaka PLCzeta cRNA. Our findings provide the first evidence that PLCzeta may be expressed in the egg, rather than the sperm, in some vertebrate species, and that its mechanism of action and physiologic role at fertilization may differ in different vertebrate species.
Authors:
Kevin Coward; Chris P Ponting; Nan Zhang; Claire Young; Chang-Jen Huang; Chih-Ming Chou; Junaid Kashir; Rafael A Fissore; John Parrington
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-01-14
Journal Detail:
Title:  Molecular reproduction and development     Volume:  78     ISSN:  1098-2795     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-26     Completed Date:  2011-04-26     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  48-56     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Calcium / metabolism
Chickens
Female
Fish Proteins / biosynthesis*,  genetics
Gene Expression Regulation, Enzymologic / physiology*
Humans
Male
Mice
Molecular Sequence Data
Organ Specificity
Ovary / cytology,  enzymology*
Ovum / cytology,  enzymology
Phosphoinositide Phospholipase C / biosynthesis*,  genetics
Species Specificity
Takifugu / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
MC_U137761446//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Fish Proteins; EC 3.1.4.11/Phosphoinositide Phospholipase C; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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