| Identification and functional analyses of two cDNAs that encode fatty acid 9-/13-hydroperoxide lyase (CYP74C) in rice. | |
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MedLine Citation:
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PMID: 16116284 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fatty acid hydroperoxide lyase (HPL), a member of cytochrome P450 (CYP74), produces aldehydes and oxo-acids involved in plant defensive reactions. In monocots, HPL that cleaves 13-hydroperoxides of fatty acids has been reported, but HPL that cleaves 9-hydroperoxides is still unknown. To find this type of HPL, in silico screening of candidate cDNA clones and subsequent functional analyses of recombinant proteins were performed. We found that AK105964 and AK107161 (Genbank accession numbers), cDNAs previously annotated as allene oxide synthase (AOS) in rice, are distinctively grouped from AOS and 13-HPL. Recombinant proteins of these cDNAs produced in Escherichia. coli cleaved both 9- and 13-hydroperoxide of linoleic and linolenic into aldehydes, while having only a trace level of AOS activity and no divinyl ether synthase activity. Hence we designated AK105964 and AK107161 OsHPL1 and OsHPL2 respectively. They are the first CYP74C family cDNAs to be found in monocots. |
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Authors:
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Hisao Kuroda; Toshiyuki Oshima; Hirotaka Kaneda; Masachika Takashio |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 69 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-23 Completed Date: 2005-12-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1545-54 Citation Subset: IM |
Affiliation:
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Frontier Laboratories of Value Creation, SAPPORO BREWERIES LTD., Shizuoka, Japan. Hisao.Kuroda@sapporobeer.co.jp |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AK105964; AK107161 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Carbon-Oxygen Lyases / chemistry, genetics*, metabolism Cloning, Molecular Cytochrome P-450 Enzyme System / chemistry, genetics*, metabolism DNA Primers DNA, Complementary / genetics* Escherichia coli / genetics Molecular Sequence Data Oryza sativa / enzymology*, genetics Sequence Homology, Amino Acid Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Complementary; 9035-51-2/Cytochrome P-450 Enzyme System; EC 4.2.-/Carbon-Oxygen Lyases; EC 4.2.-/fatty acid 9-, 13-hydroperoxide lyase, Oryza sativa |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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