Document Detail

Identification and functional analyses of two cDNAs that encode fatty acid 9-/13-hydroperoxide lyase (CYP74C) in rice.
MedLine Citation:
PMID:  16116284     Owner:  NLM     Status:  MEDLINE    
Fatty acid hydroperoxide lyase (HPL), a member of cytochrome P450 (CYP74), produces aldehydes and oxo-acids involved in plant defensive reactions. In monocots, HPL that cleaves 13-hydroperoxides of fatty acids has been reported, but HPL that cleaves 9-hydroperoxides is still unknown. To find this type of HPL, in silico screening of candidate cDNA clones and subsequent functional analyses of recombinant proteins were performed. We found that AK105964 and AK107161 (Genbank accession numbers), cDNAs previously annotated as allene oxide synthase (AOS) in rice, are distinctively grouped from AOS and 13-HPL. Recombinant proteins of these cDNAs produced in Escherichia. coli cleaved both 9- and 13-hydroperoxide of linoleic and linolenic into aldehydes, while having only a trace level of AOS activity and no divinyl ether synthase activity. Hence we designated AK105964 and AK107161 OsHPL1 and OsHPL2 respectively. They are the first CYP74C family cDNAs to be found in monocots.
Hisao Kuroda; Toshiyuki Oshima; Hirotaka Kaneda; Masachika Takashio
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  69     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-23     Completed Date:  2005-12-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1545-54     Citation Subset:  IM    
Frontier Laboratories of Value Creation, SAPPORO BREWERIES LTD., Shizuoka, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AK105964;  AK107161
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MeSH Terms
Amino Acid Sequence
Base Sequence
Carbon-Oxygen Lyases / chemistry,  genetics*,  metabolism
Cloning, Molecular
Cytochrome P-450 Enzyme System / chemistry,  genetics*,  metabolism
DNA Primers
DNA, Complementary / genetics*
Escherichia coli / genetics
Molecular Sequence Data
Oryza sativa / enzymology*,  genetics
Sequence Homology, Amino Acid
Substrate Specificity
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 9035-51-2/Cytochrome P-450 Enzyme System; EC 4.2.-/Carbon-Oxygen Lyases; EC 4.2.-/fatty acid 9-, 13-hydroperoxide lyase, Oryza sativa

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