Document Detail

Identification of a functional AP1 element in the rat vasopressin gene promoter.
MedLine Citation:
PMID:  16543367     Owner:  NLM     Status:  MEDLINE    
Arginine vasopressin (AVP) is expressed in paraventricular, supraoptic, and suprachiasmatic nuclei of the hypothalamus, where transcription of the AVP gene is activated by various forms of stress, such as hyperosmolality, inflammation, and photic stimulation. In vasopressinergic neurons, the expression of the Fos/Jun family proteins is known to be rapidly induced after these stimuli as well. However, it is still unknown whether these proteins actually mediate AVP gene expression. In this study we examined in vitro the role of Fos/Jun protein in transcriptional regulation of the AVP gene using the BE(2)M17 neuroblastoma cell line. We found that 5'-promoter activity of the rat AVP gene (-803/+26) markedly increased when all combinations of the Fos/Jun family proteins were overexpressed. Coexpression of the cAMP-responsive element-binding protein-binding protein and steroid receptor coactivator-1a further enhanced the Fos/Jun-mediated transcription. Using site-directed mutagenesis and EMSA techniques, we identified an activation protein 1 (AP1)-like element (-134/-128; TGAATCA) in the AVP gene 5'-promoter region, which is the sole responsible site for the Fos/Jun-mediated transcription. We also found that 12-O-tetradecarbonyl phorbol 13-acetate stimulates AVP gene transcription partly via the AP1 site through the activation of ERK signaling. Together, these results suggest that a variety of Fos/Jun family member proteins stimulate transcription of the AVP gene through the AP1 site we identified. Furthermore, this effect may be activated by both protein kinase A and protein kinase C signaling pathways.
Masanori Yoshida; Yasumasa Iwasaki; Masato Asai; Shinobu Takayasu; Takafumi Taguchi; Keiichi Itoi; Kozo Hashimoto; Yutaka Oiso
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Publication Detail:
Type:  Journal Article     Date:  2006-03-16
Journal Detail:
Title:  Endocrinology     Volume:  147     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-18     Completed Date:  2006-06-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2850-63     Citation Subset:  AIM; IM    
Department of Endocrinology, Toyota Memorial Hospital, Toyota 471-8513, Japan.
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MeSH Terms
Arginine Vasopressin / genetics*
Cells, Cultured
Cyclic AMP-Dependent Protein Kinases / physiology
Extracellular Signal-Regulated MAP Kinases / physiology
Histone Acetyltransferases
MAP Kinase Signaling System / physiology
Membrane Proteins / physiology
Nuclear Receptor Coactivator 1
Phosphoproteins / physiology
Promoter Regions, Genetic*
Protein Kinase C / physiology
Response Elements
Transcription Factor AP-1 / physiology*
Transcription Factors / physiology
Reg. No./Substance:
0/Cbp protein, rat; 0/Membrane Proteins; 0/Ncoa1 protein, mouse; 0/Phosphoproteins; 0/Transcription Factor AP-1; 0/Transcription Factors; 113-79-1/Arginine Vasopressin; EC Acetyltransferases; EC Receptor Coactivator 1; EC AMP-Dependent Protein Kinases; EC Kinase C; EC Signal-Regulated MAP Kinases

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