Document Detail


Identification of follicular marker genes as pregnancy predictors for human IVF: new evidence for the involvement of luteinization process.
MedLine Citation:
PMID:  20610614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multiple pregnancy represents an important health risk to both mother and child in fertility treatment. To reduce a high twin rate, restriction to one embryo transfer is needed. Morphological evaluation methods for predicting embryo viability has significant limitations. Tight communication exists between the follicular cells (FCs) and the oocyte; therefore, developmental competence may be determined by markers expressed in the surrounding FCs. In this study, cells were recovered on a per-follicle basis by individual follicle puncture. Hybridization analysis using a custom-made complementary DNA microarray containing FC transcripts was performed. Genes expressed in FCs associated with good morphological transferred embryos were identified from follicles associated with a pregnancy outcome (pregnancy group) or no pregnancy (non-pregnancy group). Ten candidates from the Pregnancy group and three from the Non-pregnancy group were validated by quantitative RT-PCR. The best predictors associated with pregnancy were UDP-glucose pyrophosphorylase-2 and pleckstrin homology-like domain, family A, member 1. Genes assessment showed no significant candidate genes associated with non-pregnancy outcome, but GA-binding protein transcription factor beta1 showed a tendency to be potentially more expressed in the non-pregnancy group. These markers could be related to granulosa luteinization process and could be used to improve embryo selection for successful single embryo transfer.
Authors:
Mélanie Hamel; Isabelle Dufort; Claude Robert; Marie-Claude Léveillé; Arthur Leader; Marc-André Sirard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-07
Journal Detail:
Title:  Molecular human reproduction     Volume:  16     ISSN:  1460-2407     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-21     Completed Date:  2010-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  548-56     Citation Subset:  IM    
Affiliation:
Département des Sciences Animales, Centre de Recherche en Biologie de la Reproduction (CRBR), Université Laval, Montréal, QC, Canada.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Female
Fertilization in Vitro / methods*
Forecasting
Genetic Markers / genetics*
Humans
Luteinization / metabolism*
Molecular Sequence Data
Ovarian Follicle / metabolism*
Pregnancy
Pregnancy Outcome
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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