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Identification of flavonoids with trypsin inhibitory activity extracted from orange peel and green tea leaves.
MedLine Citation:
PMID:  23124490     Owner:  NLM     Status:  Publisher    
BACKGROUND: Orange peel (Citrus sinensis) and green tea (Camellia sinensis) leaves, rich sources of food flavonoids, were analyzed for their trypsin inhibitory potential. Hesperetin, rutin and hesperidin from orange peel, and catechin from green tea leaves, were isolated and their chemical structures were analyzed. All four compounds were evaluated for their trypsin inhibitory potential. RESULTS: Among all the isolated compounds, rutin exhibited the highest protease inhibition activity (75.4 ± 0.9%) with IC(50)  = 16 ± 2 µmol L(-1) , followed by catechin (65.3 ± 1.4%; IC(50)  = 83 ± 9 µmol L(-1) ), hesperetin (62.1 ± 1.3%; IC(50)  = 104 ± 12 µmol L(-1) ) and hesperidin (59.7 ± 1.1%; IC(50)  = 127 ± 14 µmol L(-1) ). Lineweaver-Burk and Dixon plots and their secondary replots indicated that all four compounds possessed non-competitive inhibition. The K(i) values of hesperetin, rutin, hesperidin and catechin were calculated as 90.2 ± 1.1, 17.5 ± 0.6, 84.2 ± 1.5 and 65.1 ± 1.5 µmol L(-1) respectively. CONCLUSION: The present results suggest that the four isolated flavonoids can be used as a supplement in food for the treatment of pathologies associated with the degradation of a specific protein. © 2012 Society of Chemical Industry.
Durre Shahwar; Muhammad A Raza; Atta-Ur-Rahman
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-11
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  -     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-11-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Society of Chemical Industry.
Research Lab II, Department of Chemistry, Government College University, Lahore, 54000, Pakistan.
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