| Identification of flavonoid metabolites after oral administration to rats of a Ginkgo biloba extract. | |
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MedLine Citation:
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PMID: 8925077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An extract of Ginkgo biloba leaves (EGb) was administered by gastric probe to Wistar female rats, and urine and faeces samples were collected for 5 days and whole blood samples were withdrawn every 30 min for 6 h. After purification with SPE C18 cartridges, the samples were analysed by reversed-phase LC-diode array detection (LC-DAD) for residual flavonoid glycosides, aglycones and metabolites. No glycosides or aglycones were detected in urine, faeces or blood and extensive degradation of EGb flavonoids within 24 h was detected. Among the seven different phenylalkyl acids detected by LC-DAD, 3,4-dihydroxyphenylacetic acid (I), hippuric acid (II), 3-hydroxyphenylacetic acid (III), homovanillic acid (IV) and benzoic acid (VII) were directly confirmed by on-line mass spectrometry using an electrospray interface (ES-MS). Peaks V and VI needed to be collected and separately examined and they were found to be 3-(4-hydrophenyl)propionic acid and 3-(3-hydrophenyl)propionic acid, respectively. As further evidence, the identity of metabolites I, II, III, IV, V and VII was confirmed by co-chromatography with authentic standards. |
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Authors:
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P G Pietta; C Gardana; P L Mauri; R Maffei-Facino; M Carini |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 673 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-11-13 Completed Date: 1996-11-13 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 75-80 Citation Subset: IM |
Affiliation:
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CNR-ITBA, Milan, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3,4-Dihydroxyphenylacetic Acid
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analysis Administration, Oral Animals Benzoates / analysis Benzoic Acid Chromatography, High Pressure Liquid Feces / chemistry Female Flavonoids / metabolism* Flavonols Free Radical Scavengers / metabolism* Hippurates / analysis Homovanillic Acid / analysis Mass Spectrometry Phenylacetates / analysis Plant Extracts / administration & dosage, metabolism* Propionic Acids / analysis Rats Rats, Wistar Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Benzoates; 0/Flavonoids; 0/Flavonols; 0/Free Radical Scavengers; 0/Hippurates; 0/Phenylacetates; 0/Plant Extracts; 0/Propionic Acids; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 306-08-1/Homovanillic Acid; 495-69-2/hippuric acid; 621-37-4/3-hydroxyphenylacetic acid; 65-85-0/Benzoic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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