Document Detail

Identification of flavonoid metabolites after oral administration to rats of a Ginkgo biloba extract.
MedLine Citation:
PMID:  8925077     Owner:  NLM     Status:  MEDLINE    
An extract of Ginkgo biloba leaves (EGb) was administered by gastric probe to Wistar female rats, and urine and faeces samples were collected for 5 days and whole blood samples were withdrawn every 30 min for 6 h. After purification with SPE C18 cartridges, the samples were analysed by reversed-phase LC-diode array detection (LC-DAD) for residual flavonoid glycosides, aglycones and metabolites. No glycosides or aglycones were detected in urine, faeces or blood and extensive degradation of EGb flavonoids within 24 h was detected. Among the seven different phenylalkyl acids detected by LC-DAD, 3,4-dihydroxyphenylacetic acid (I), hippuric acid (II), 3-hydroxyphenylacetic acid (III), homovanillic acid (IV) and benzoic acid (VII) were directly confirmed by on-line mass spectrometry using an electrospray interface (ES-MS). Peaks V and VI needed to be collected and separately examined and they were found to be 3-(4-hydrophenyl)propionic acid and 3-(3-hydrophenyl)propionic acid, respectively. As further evidence, the identity of metabolites I, II, III, IV, V and VII was confirmed by co-chromatography with authentic standards.
P G Pietta; C Gardana; P L Mauri; R Maffei-Facino; M Carini
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  673     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-11-13     Completed Date:  1996-11-13     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM    
CNR-ITBA, Milan, Italy.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / analysis
Administration, Oral
Benzoates / analysis
Benzoic Acid
Chromatography, High Pressure Liquid
Feces / chemistry
Flavonoids / metabolism*
Free Radical Scavengers / metabolism*
Hippurates / analysis
Homovanillic Acid / analysis
Mass Spectrometry
Phenylacetates / analysis
Plant Extracts / administration & dosage,  metabolism*
Propionic Acids / analysis
Rats, Wistar
Reg. No./Substance:
0/Benzoates; 0/Flavonoids; 0/Flavonols; 0/Free Radical Scavengers; 0/Hippurates; 0/Phenylacetates; 0/Plant Extracts; 0/Propionic Acids; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 306-08-1/Homovanillic Acid; 495-69-2/hippuric acid; 621-37-4/3-hydroxyphenylacetic acid; 65-85-0/Benzoic Acid

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