Document Detail


Identification of fibronectin in chicken thrombocytes.
MedLine Citation:
PMID:  1606623     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell attachment and spreading of chicken embryo fibroblasts and three mammalian cell lines were promoted by using thrombocyte secretion products (TSP) prepared from chicken thrombocytes which are analogous to mammalian platelets. The cell attachment activity following TSP treatment was interfered with by the addition of a synthetic peptide containing the RGD sequence from the cell attachment region of fibronectin (FN). Cell attachment on TSP-coated plates was inhibited by the addition of anti-chicken FN rabbit antiserum, but not by anti-chicken vitronectin (VN) rabbit antiserum. By immunoblotting, immunofluorescent test and sensitive enzyme linked immunosorbent assay using an anti-chicken FN antiserum, the presence of FN in thrombocytes and the release of FN from the cells were demonstrated. The release of FN from thrombocytes was partially induced by the in vitro cultivation of the cells and was further promoted by treatment of the cells with thrombin.
Authors:
H Horiuchi; M Hayashi; H Matsuda; M Murata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell structure and function     Volume:  17     ISSN:  0386-7196     ISO Abbreviation:  Cell Struct. Funct.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-07-22     Completed Date:  1992-07-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7608465     Medline TA:  Cell Struct Funct     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  93-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Blood Platelets / chemistry*,  metabolism
Blood Proteins / analysis*
Cell Adhesion Molecules / blood*
Cells, Cultured
Chick Embryo
Chickens
Fibronectins / blood*
Molecular Sequence Data
Oligopeptides / blood
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Cell Adhesion Molecules; 0/Fibronectins; 0/Oligopeptides; 99896-85-2/arginyl-glycyl-aspartic acid

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