Document Detail

Identification of the feline CD63 homologue using retrovirus-mediated expression cloning.
MedLine Citation:
PMID:  15010227     Owner:  NLM     Status:  MEDLINE    
Recently, we combined a retrovirus-mediated expression cloning with a simple screening method using non-adherent cells and panning [Anal. Biochem. 315 (2003) 138]. In this study, we applied this method to identify the antigen recognized by an uncharacterized monoclonal antibody raised against a feline cell line, and identified it as the feline homologue of CD63. This simple method is useful for characterizing unknown antibodies that recognize cell surface molecules. Furthermore, the monoclonal antibody identified as an anti-feline CD63 antibody will be useful for studying feline molecular function(s).
Yumiko Sakurai; Masayuki Shimojima; Takayuki Miyazawa; Kouta Masuoka; Yukinobu Tohya; Hiroomi Akashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  98     ISSN:  0165-2427     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-10     Completed Date:  2004-05-20     Revised Date:  2010-10-20    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  185-91     Citation Subset:  IM    
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / immunology
Antigens, CD / genetics*,  immunology*
Base Sequence
Cats / genetics*,  immunology*
Cell Line, Tumor
Cloning, Molecular / methods
Flow Cytometry / veterinary
Fluorescent Antibody Technique, Indirect / veterinary
Gene Library
Genetic Vectors
Molecular Sequence Data
Moloney murine leukemia virus / genetics
Platelet Membrane Glycoproteins / genetics*,  immunology*
Sequence Alignment
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/CD63 antigen; 0/Platelet Membrane Glycoproteins

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