Document Detail


Identification and evaluation of anti hepatitis C virus phytochemicals from Eclipta alba.
MedLine Citation:
PMID:  23026306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ETHNOPHARMACOLOGICAL RELEVANCE: Eclipta alba, traditionally known as bhringraj, has been used in Ayurvedic medicine for more than 1000 years in India. It is used for the treatment of infective hepatitis, liver cirrhosis, liver enlargement and other ailments of liver and gall bladder in India. The aim of this study was to evaluate anti-hepatitis C virus activity present in the Eclipta alba extract, perform bioassay based fractionation and identify anti-HCV phytochemicals from the active fractions.
MATERIALS AND METHODS: Identification of active compounds was performed by bio-activity guided fractionation approach. Active isolates were separated by the combination of silica gel chromatography and preparative scale reverse phase HPLC. Eclipta alba extract and its isolates were examined for their ability to inhibit HCV replicase (HCV NS5B) activity in vitro and HCV replication in a cell culture system carrying replicating HCV subgenomic RNA replicon. The purified isolates were also examined for their binding affinity to HCV replicase by fluorescence quenching and their cytotoxicity by MTT assay.
RESULTS: Eclipta alba extract strongly inhibited RNA dependent RNA polymerase (RdRp) activity of HCV replicase in vitro. In cell culture system, it effectively inhibited HCV replication which resulted in reduced HCV RNA titer and translation level of viral proteins. Bioassay-based fractionations of the extracts and purification of anti-HCV phytochemicals present in the active fractions have identified three compounds, wedelolactone, luteolin, and apigenin. These compounds exhibited dose dependent inhibition of HCV replicase in vitro, and anti-HCV replication activity in the cell culture system
CONCLUSION: Eclipta alba extract and phytochemicals isolated from active fractions display anti-HCV activity in vitro and in cell culture system. The standardized Eclipta alba extract or its isolates can be used as an effective alternative and complementary treatment against HCV.
Authors:
Dinesh Manvar; Mahesh Mishra; Suriender Kumar; Virendra N Pandey
Related Documents :
24198226 - Advanced hiv disease at entry into hiv care and initiation of antiretroviral therapy du...
24668726 - Truncated cxcl10 is associated with failure to achieve spontaneous clearance of acute h...
23221626 - 3-hydroxyphthalic anhydride-modified human serum albumin as a microbicide candidate inh...
7560976 - Methodology for hiv disinfectant testing.
6785286 - Identification and detection of long incubation non-a, non-b hepatitis virus and associ...
16868706 - Survival and morbidity of hiv patients on hemodialysis and peritoneal dialysis: one cen...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-09-28
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  144     ISSN:  1872-7573     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-05-09     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  545-54     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ireland Ltd.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antiviral Agents / isolation & purification,  pharmacology*
Cell Line, Tumor
Eclipta*
Hepacivirus / drug effects*,  physiology
Humans
Plant Extracts / pharmacology*
RNA Replicase / metabolism
RNA, Viral / genetics
Virus Replication / drug effects
Grant Support
ID/Acronym/Agency:
AI073703/AI/NIAID NIH HHS; DK083560/DK/NIDDK NIH HHS; R21 AI073703/AI/NIAID NIH HHS; R21 DK083560/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antiviral Agents; 0/Plant Extracts; 0/RNA, Viral; EC 2.7.7.48/RNA Replicase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Induction of quinone oxidoreductase 1 enzyme by Rhazya stricta through Nrf2-dependent mechanism.
Next Document:  Antimicrobial assays of three native British plants used in Anglo-Saxon medicine for wound healing f...