Document Detail


Identification of estrogen receptors in granulosa cells of immature rats.
MedLine Citation:
PMID:  6679947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nuclear and cytoplasmic binding sites for estradiol (E2-17 beta) in granulosa cells of immature rats were characterized. These binding sites for estrogen were high affinity, low capacity with an affinity constant (Kd) of 1.9 X 10(-10)M (binding capacity, Ro = 80 pM) for nuclear sites and a Kd = 3.5 X 10(-10) M (Ro = 45 pM) for cytosol sites. Binding was specific for biologically active estrogens. The estrogen receptor in granulosa cells is a protein and heat-labile as treatment with protease or pre-incubation at 37 degrees C for 1 h significantly diminished binding. RNase and DNase had no effect on estrogen binding. Sedimentation coefficients for nuclear and cytosol binding components were 5S and 8S respectively, similar to values obtained with uteri. Finally, translocation was demonstrated after a s.c. injection of E2-17 beta. Forty-five minutes post-injection, cytosol binding sites for estradiol were depleted concomitant with accumulation of nuclear binding sites. We concluded that granulosa cells of immature rats have binding sites specific for estradiol which have characteristics similar to the classical estrogen receptor in uteri.
Authors:
S A Tonetta; J J Ireland
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Steroids     Volume:  42     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1984-12-07     Completed Date:  1984-12-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  427-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Cell Nucleus / metabolism
Centrifugation, Density Gradient
Cytosol / metabolism
Estradiol / metabolism*
Female
Granulosa Cells / metabolism*
Kinetics
Rats
Rats, Inbred Strains
Receptors, Estrogen / metabolism*
Sucrose
Temperature
Uterus / metabolism
Chemical
Reg. No./Substance:
0/Receptors, Estrogen; 50-28-2/Estradiol; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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