Document Detail


Identification of endogenous retinoids, enzymes, binding proteins, and receptors during early postimplantation development in mouse: important role of retinal dehydrogenase type 2 in synthesis of all-trans-retinoic acid.
MedLine Citation:
PMID:  10753524     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Specific combinations of nuclear retinoid receptors acting as ligand-inducible transcription factors mediate the essential role of retinoids in embryonic development. Whereas some data exist on the expression of these receptors during early postimplantation development in mouse, little is known about the enzymes controlling the production of active ligands for the retinoid receptors. Furthermore, at early stages of mouse development virtually no data are available on the presence of endogenous retinoids. In the present study we have used a recently developed high-performance liquid chromatographic (HPLC) technique to identify endogenous retinoids in mouse embryos down to the egg cylinder stage. All-trans-retinoic acid, a ligand for the retinoic acid receptors, was detected in embryos dissected as early as 7.5 dpc (i.e., a combination of midstreak until late allantoic bud stage embryos). At these stages, we detected mRNA coding for all the retinoid receptors, retinoid binding proteins, and two enzymes able to convert retinol to retinal (retinol dehydrogenase 5 (RDH5) and alcohol dehydrogenase 4 (ADH4)). We also detected retinal dehydrogenase type 2 (RALDH2), an enzyme capable of oxidising the final step in the all-trans-retinoic acid synthesis. In egg cylinder stage mouse embryos no all-trans-retinoic acid was detected. However, at this stage its precursor all-trans-retinal was present. In accordance with these HPLC observations, RDH5 and ADH4 were expressed, but no transcripts coding for enzymes that oxidise retinal to retinoic acid. Therefore, our results suggest that RALDH2 is a key regulator in initiating retinoic acid synthesis sometime between the mid-primitive streak stage and the late allantoic bud stage in mouse embryos.
Authors:
S M Ulven; T E Gundersen; M S Weedon; V O Landaas; A K Sakhi; S H Fromm; B A Geronimo; J O Moskaug; R Blomhoff
Related Documents :
11093824 - Synergistic induction of apoptosis of neuroblastoma by fenretinide or cd437 in combinat...
8709094 - Discovery of novel retinoic acid receptor agonists having potent antiproliferative acti...
1655814 - Differences in the action and metabolism between retinol and retinoic acid in b lymphoc...
9302644 - Elastin expression is up-regulated by retinoic acid but not by retinol in chick embryon...
22823344 - Effects of prenatal and/or postnatal (maternal and/or child) folic acid supplementation...
3948404 - An enzymatic method for calibration of serum lipase assays.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  220     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-18     Completed Date:  2000-05-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  379-91     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
Institute for Nutrition Research, University of Oslo, Blindern, Oslo, 0316, Norway.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aldehyde Oxidoreductases / physiology*
Animals
Chromatography, High Pressure Liquid
Embryo, Mammalian / metabolism,  physiology
Mass Spectrometry
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
RNA, Messenger / metabolism
Retinal Dehydrogenase
Retinoids / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Time Factors
Tretinoin / metabolism*
Vitamin A / metabolism
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Retinoids; 11103-57-4/Vitamin A; 302-79-4/Tretinoin; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.36/Retinal Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Two pathways of maternal RNA localization at the posterior-vegetal cytoplasm in early ascidian embry...
Next Document:  Characterization of polo-like kinase 1 during meiotic maturation of the mouse oocyte.