Document Detail

Identification of endocrine cell populations expressing the AT1B subtype of angiotensin II receptors in the anterior pituitary.
MedLine Citation:
PMID:  9886859     Owner:  NLM     Status:  MEDLINE    
Angiotensin II (Ang II) participates in the regulation of anterior pituitary hormone secretion by acting either directly on the anterior pituitary or indirectly on the hypothalamus. When applied directly on pituitary cells, Ang II increases both ACTH and PRL secretion and has also been reported to affect GH secretion. Three distinct subtypes of Ang II receptors (AT1A, AT1B, and AT2) have been identified; they are unequally distributed and differently regulated in various tissues. We have previously demonstrated that only AT1A receptors are present in the hypothalamus while anterior pituitary cells express predominantly the AT1B subtype. Using in situ hybridization in combination with immunohistochemistry, the aim of the present study was to identify the phenotype of the endocrine cell expressing AT1B receptor messenger RNA (mRNA) in the anterior pituitary of adult male Sprague-Dawley rats. Expression of AT1B receptor mRNA was present in 33.9 +/- 1.0% of anterior pituitary cells. AT1B mRNA is predominantly expressed by lactotropes (78.2 +/- 2.1% of AT1B mRNA-expressing cells) and to a lower degree by corticotropes (18.3 +/- 2.1%) and is not detectable in somatotropes, mammosomatotropes, gonadotropes, or thyrotropes. These results indicate that in adult male rats, Ang II, which has been shown to be synthesized in gonadotropes, can directly stimulate PRL and ACTH release from lactotropes and corticotropes through activation of AT1B receptors. As only 53.8 +/- 2.7% of lactotropes and 23.6 +/- 2.8% of corticotropes expressed AT1B mRNA, our findings suggest a functional heterogeneity of both cell types regarding their sensitivity to Ang II.
Z Lenkei; A M Nuyt; D Grouselle; P Corvol; C Llorens-Cortès
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  140     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-01-28     Completed Date:  1999-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  472-7     Citation Subset:  AIM; IM    
INSERM U-36, Collège de France, Paris.
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MeSH Terms
Adrenocorticotropic Hormone / metabolism
In Situ Hybridization
Pituitary Gland, Anterior / chemistry,  cytology*
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptors, Angiotensin / biosynthesis*,  genetics
Reg. No./Substance:
0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 0/Receptors, Angiotensin; 9002-60-2/Adrenocorticotropic Hormone

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