Document Detail


Identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by HPLC-ESI-MS.
MedLine Citation:
PMID:  16028988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ellagic acid, ellagic acid glycosides, and ellagitannins found in various fruits and nuts, including muscadine grape, are reported to have potential health-promoting benefits and antioxidant properties. This study isolated and identified several ellagic acid derivatives present in muscadine grapes and determined their relative antioxidant properties (AOX). Compounds were extracted from grape skins and pulp using methanol, and the solvent was evaporated. Isolates were dissolved in citric acid buffer (pH 3.5) and absorbed onto C18 cartridges. Nonretained polyphenolics were collected separately and again partitioned from Sephadex LH-20, whereas retained polyphenolics were first eluted with ethyl acetate followed by methanol. Ellagic acid derivatives were identified on the basis of UV and mass spectra, and the presence of ellagitannins was confirmed by a significant increase in free ellagic acid with HPLC followed by acid hydrolysis. Muscadine grapes contained phenolic acids, flavonols, anthocyanins, ellagic acid, and numerous ellagic acid derivatives. AOX varied in the order ethyl acetate > methanol > C18 nonretained fractions; each correlated to both total phenolics (r = 0.90) and total ellagic acid (r = 0.99) contents. Results of this study revealed previously unidentified ellagic acid derivatives in muscadine grapes.
Authors:
Joon-Hee Lee; Jodie V Johnson; Stephen T Talcott
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-20     Completed Date:  2005-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6003-10     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110370, Gainesville, Florida 32611, USA.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / analysis
Chromatography, High Pressure Liquid*
Ellagic Acid / analysis*
Flavonoids / analysis*
Fruit / chemistry*
Glycosides / analysis
Hydrolyzable Tannins / analysis
Phenols / analysis*
Spectrometry, Mass, Electrospray Ionization*
Vitis / chemistry*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Flavonoids; 0/Glycosides; 0/Hydrolyzable Tannins; 0/Phenols; 0/polyphenols; 476-66-4/Ellagic Acid

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