Document Detail


Identification of each human chromosome with a modified Giemsa stain.
MedLine Citation:
PMID:  4105987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the pattern is quite similar to that obtained with quinacrine mustard fluorescence staining. Certain regions, such as the paracentric constrictions in chromosomes Al and C9, and the distal end of the long arm of the Y chromosome stain differently with the Giemsa 9 technique. The technique is considerably simpler than the quinacrine mustard fluorescence technique and identification of homologs is also easier than in cells stained by the latter.
Authors:
S R Patil; S Merrick; H A Lubs
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  173     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1971 Aug 
Date Detail:
Created Date:  1971-11-17     Completed Date:  1971-11-17     Revised Date:  2011-12-13    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  821-2     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Buffers
Chromosomes*
Fluorescent Dyes
Humans
Hydrogen-Ion Concentration
Karyotyping*
Methods
Quinacrine
Staining and Labeling*
Chemical
Reg. No./Substance:
0/Buffers; 0/Fluorescent Dyes; 83-89-6/Quinacrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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