| Identification and distribution of photoreceptor subtypes in the neotenic tiger salamander retina. | |
| | |
MedLine Citation:
|
PMID: 9839981 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The neotenic tiger salamander retina is a major model system for the study of retinal physiology and circuitry, yet there are unresolved issues regarding the organization of the photoreceptors and the photoreceptor mosaic. The rod and cone subtypes in the salamander retina were identified using a combination of morphological and immunocytochemical markers for specific rod and cone opsin epitopes. Because the visual pigment mechanisms present in the tiger salamander retina are well characterized and the antibodies employed in these studies are specific for particular rod and cone opsin epitopes, we also were able to identify the spectral class of the various rod and cone subtypes. Two classes of rods corresponding to the "red" and "green" rods previously reported in amphibian retinas were identified. In serial semithin section analyses, rods and cones comprised 62.4+/-1.4% and 37.6+/-1.4% of all photoreceptors, respectively. One rod type comprising 98.0+/-0.7% of all rods showed the immunological and morphological characteristics of "red" rods, which are maximally sensitive to middle wavelengths. The second rod subtype comprised 2.0+/-0.7% of all rods and possessed the immunological and morphological characteristics of "green" rods, which are maximally sensitive to short wavelengths. By morphology four cone types were identified, showing three distinct immunological signatures. Most cones (84.8+/-1.5% of all cones), including most large single cones, the accessory and principal members of the double cone, and some small single cones, showed immunolabeling by antisera that recognize long wavelength-sensitive cone opsins. A subpopulation of small single cones (8.4+/-1.7% of all cones) showed immunolabeling for short wavelength-sensitive cone opsin. A separate subpopulation of single cones which included both large and small types (6.8+/-1.4% of all cones) was identified as the UV-Cone population and showed immunolabeling by antibodies that recognize rod opsin epitopes. Analysis of flatmounted retinas yielded similar results. All photoreceptor types appeared to be distributed in all retinal regions. There was no obvious crystalline organization of the various photoreceptor subtypes in the photoreceptor mosaic. |
| | |
Authors:
|
D M Sherry; D D Bui; W J Degrip |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Visual neuroscience Volume: 15 ISSN: 0952-5238 ISO Abbreviation: Vis. Neurosci. Publication Date: 1998 Nov-Dec |
Date Detail:
|
Created Date: 1999-02-26 Completed Date: 1999-02-26 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8809466 Medline TA: Vis Neurosci Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1175-87 Citation Subset: IM |
Affiliation:
|
College of Optometry, University of Houston, TX 77204-6052, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Image Processing, Computer-Assisted Immunohistochemistry Photography Retina / cytology* Retinal Cone Photoreceptor Cells / cytology* Retinal Rod Photoreceptor Cells / cytology* Urodela / anatomy & histology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A phenomenological model of visually evoked spike trains in cat geniculate nonlagged X-cells.
Next Document: Perfusion system components release agents that distort functional properties of rod cyclic nucleoti...