Document Detail

Identification of differentially expressed genes in clear cell renal cell carcinoma by analysis of full-length enriched cDNA library.
MedLine Citation:
PMID:  16453177     Owner:  NLM     Status:  MEDLINE    
Renal cell carcinoma (RCC) is the most common malignancy in adult kidney, and accounts for 3% of malignancies worldwide with increasing incidence. Clear cell RCC (ccRCC) is the major type in RCC. Resection by surgery is the main treatment because the response of ccRCC to traditional therapies is very poor. To identify the tumor-associated genes for better understanding the molecular mechanism of ccRCC, the full-length enriched cDNA libraries of ccRCC and normal kidney tissues were constructed by the oligo-capping method. Nucleotide sequences of the cDNA libraries of ccRCC and normal kidney tissues were sequenced. From the sequence analysis of 19,425 and 12,400 clones of ccRCC and normal kidney tissues, 4356 and 3055 genes were identified, respectively. By comparing the gene-expression patterns of ccRCC and normal tissues, the up- or down-regulated genes were identified. Among these identified genes, the differential expression of annexin A2 and argininosuccinate synthetase genes were further confirmed by quantitative real-time PCR and Western blot analysis.
Sai-Wen Tang; Wen-Hsin Chang; Yu-Wei Chao; Chung-Yei Lin; Hsiao-Fen Chen; Yen-Han Lai; Bo-Wen Zhan; Yih-Ching Su; Sen-Wen Jane; Yu-Chi Chen; Chyong-Ing Hsu; Wei-Chou Lin; Kao-Chung Wang; Ming-Kuen Lai; Jung-Yaw Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical science     Volume:  13     ISSN:  1021-7770     ISO Abbreviation:  J. Biomed. Sci.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-28     Completed Date:  2006-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421567     Medline TA:  J Biomed Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  233-40     Citation Subset:  IM    
Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
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MeSH Terms
Annexin A2 / genetics
Argininosuccinate Synthase / genetics
Carcinoma, Renal Cell / genetics*
Gene Expression Profiling*
Gene Expression Regulation, Neoplastic*
Gene Library
Neoplasm Proteins / genetics
Reg. No./Substance:
0/Annexin A2; 0/Neoplasm Proteins; EC Synthase

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