Document Detail


Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress.
MedLine Citation:
PMID:  16946266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mycobacterium tuberculosis under stress stores triacylglycerol (TG). There are 15 genes in M. tuberculosis that belong to a novel family of TG synthase genes (tgs), but it is not known which of them is responsible for this accumulation of TG. In this paper, it is reported that M. tuberculosis H37Rv accumulated TG under acidic, static or hypoxic growth conditions, or upon treatment with NO, whereas TG accumulation was drastically reduced in the tgs1 (Rv3130c) disrupted mutant. Complementation with tgs1 restored this TG accumulation. C(26) was a major fatty acid in this TG, indicating that the TGS1 gene product uses C(26) fatty acid, which is known to be produced by the mycobacterial fatty acid synthase. TGS1 expressed in Escherichia coli preferred C(26 : 0)-CoA for TG synthesis. If TG storage is needed for the long-term survival of M. tuberculosis under dormant conditions, the tgs1 product could be a suitable target for antilatency drugs.
Authors:
Tatiana D Sirakova; Vinod S Dubey; Chirajyoti Deb; Jaiyanth Daniel; Tatiana A Korotkova; Bassam Abomoelak; Pappachan E Kolattukudy
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  152     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-01     Completed Date:  2006-10-26     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  2717-25     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anoxia
Diacylglycerol O-Acyltransferase / genetics*
Escherichia coli / genetics
Genes, Bacterial*
Hydrogen-Ion Concentration
Mutation
Mycobacterium tuberculosis / drug effects,  enzymology*,  genetics*,  growth & development,  metabolism*
Nitric Oxide / pharmacology
Triglycerides / biosynthesis*,  genetics
Grant Support
ID/Acronym/Agency:
AI35272/AI/NIAID NIH HHS; AI46582/AI/NIAID NIH HHS; R01 AI046582/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Triglycerides; 31C4KY9ESH/Nitric Oxide; EC 2.3.1.20/Diacylglycerol O-Acyltransferase
Comments/Corrections

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