Identification and determination of carboxylic acids in food samples using 2-(2-(anthracen-10-yl)-1H-phenanthro[9,10-d]imidazol-1-yl)ethyl 4-methylbenzenesulfonate (APIETS) as labeling reagent by HPLC with FLD and APCI/MS.

Identification and determination of carboxylic acids in food samples using 2-(2-(anthracen-10-yl)-1H-phenanthro[9,10-d]imidazol-1-yl)ethyl 4-methylbenzenesulfonate (APIETS) as labeling reagent by HPLC with FLD and APCI/MS.

MedLine Citation:	PMID: 21726743 Owner: NLM Status: In-Data-Review
Abstract/OtherAbstract:	A new labeling reagent for carboxylic acids, 2-(2-(anthracen-10-yl)-1H-phenanthro[9,10-d]imidazol-1-yl)ethyl 4-methylbenzenesulfonate (APIETS) has been designed and synthesized. It was used to label eight fatty acids (lauric acid, myristic acid, palmitic acid, stearic acid, arachidic acid, oleic acid, linoleic acid and linolenic acid) and four hydroxy pentacyclic triterpene acids (oleanolic acid, ursolic acid, betulinic acid and maslinic acid), successfully. APIETS could easily and quickly label carboxylic acids in the presence of K(2)CO(3) catalyst at 85°C for 35min in N,N-dimethylformamide solvent. The carboxylic acids derivatives were separated on a C(8) reversed-phase column with gradient elution and fluorescence detection at λ(ex)/λ(em)=315/435nm. Identification of these derivatives was carried out by online mass spectrometry with atmospheric pressure chemical ionization in positive ion mode. The detection limits obtained were 13.37-30.26fmol (signal-to-noise ratio of 3). The proposed method has been applied to the quantification of carboxylic acids in sultana raisin (Thompson seedless), hawthorn flake (Crataegus pinnatifida Bge.), Lycium barbarum seed oil and Microula sikkimensis seed oil with recoveries over 95.3%. It has been demonstrated that APIETS is a prominent labeling reagent for determining carboxylic acids with high performance liquid chromatography.

Authors:	Zhiwei Sun; Jinmao You; Cuihua Song; Lian Xia
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Publication Detail:	Type: Journal Article Date: 2011-05-18
Journal Detail:	Title: Talanta Volume: 85 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2011 Aug
Date Detail:	Created Date: 2011-07-05 Completed Date: - Revised Date: -
Medline Journal Info:	Nlm Unique ID: 2984816R Medline TA: Talanta Country: England
Other Details:	Languages: eng Pagination: 1088-99 Citation Subset: IM
Copyright Information:	Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:	Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, PR China; Northwest Plateau Institute of Biology, Chinese Academy of Science, Xining, PR China; Graduate University of the Chinese Academy of Science, Beijing, PR China.
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