Document Detail


Identification and cytogenetic analysis of an abnormal pig chromosome for flow cytometry and sorting.
MedLine Citation:
PMID:  8216615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For cytogenetics of pig (Sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to establish DNA-libraries, or to produce DNA-probes. Flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are described. Flow sorting of the largest chromosomes of these cells was performed. After sorting the chromosomes still had a well preserved morphology and were identified microscopically by G-banding. The quality of the band pattern of the sorted chromosomes was compatible to that of isolated chromosomes not subjected to flow cytometry. The sorted fraction showed an enrichment of chromosome 6/15 and chromosome 1 which have quantitatively about the same integrated fluorescence intensity. Slit scan flow analysis was performed to discriminate these two chromosomes. Metacentric and submetacentric chromosomes were analyzed according to their bimodal slit scan profiles. Profiles of the largest chromosomes were distinguished by their different centromeric indices. Two groups were interpreted as the normal chromosome 1 and the translocation chromosome 6/15.
Authors:
M Hausmann; C P Popescu; J Boscher; D Kerboeuf; J Dölle; C Cremer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zeitschrift für Naturforschung. C, Journal of biosciences     Volume:  48     ISSN:  0939-5075     ISO Abbreviation:  Z. Naturforsch., C, J. Biosci.     Publication Date:    1993 Jul-Aug
Date Detail:
Created Date:  1993-12-01     Completed Date:  1993-12-01     Revised Date:  2009-11-04    
Medline Journal Info:
Nlm Unique ID:  8912155     Medline TA:  Z Naturforsch C     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  645-53     Citation Subset:  IM    
Affiliation:
Laboratoire de Cytogénétique, INRA, Jouy-en-Josas, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Chromosome Aberrations*
Chromosome Banding
Chromosome Disorders*
Chromosome Mapping*
Fibroblasts / cytology
Flow Cytometry / methods
Karyotyping
Swine / genetics*
Translocation, Genetic*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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