Document Detail


MedLine Citation:
PMID:  11848568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A duplex polymerase chain reaction (PCR) was developed to identify the milk of bovine and buffalo species in cheese products, particularly in mozzarella cheese, a typical Italian cheese made from buffalo's milk. Two sets of primers were designed on the basis of the alignment of the sequence codifying mitochondrial cyt b available in the GenBank database. The primers proved to be species-specific, giving rise to 279-bp (bovine) and 192-bp (buffalo) amplified fragments. Since the amplification conditions for bovine and buffalo primers were identical, a duplex PCR was successfully applied to identify the two species in a single reaction step. This technique, when used to test cheese products from the retail trade, allowed the detection of partial or even total substitution of cow's milk for buffalo's milk, in some cases in samples of cheese misleadingly labeled "pure buffalo" mozzarella.
Authors:
M T Bottero; T Civera; A Anastasio; R M Turi; S Rosati
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  65     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-18     Completed Date:  2002-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  362-6     Citation Subset:  IM    
Affiliation:
Dipartimento di Patologia Animale, Università degli Studi Torino, Italy. bottero@veter.unito.it
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF371961;  AF371962
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Buffaloes
Cattle
Cheese / analysis*
Food Labeling
Gene Amplification
Milk / chemistry
Molecular Sequence Data
Polymerase Chain Reaction / methods
Sequence Alignment
Species Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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