Document Detail


Identification of a conserved protein involved in anaerobic unsaturated fatty acid synthesis in Neiserria gonorrhoeae: implications for facultative and obligate anaerobes that lack FabA.
MedLine Citation:
PMID:  21895795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcriptome analysis of the facultative anaerobe, Neisseria gonorrhoeae, revealed that many genes of unknown function were induced under anaerobic conditions. Mutation of one such gene, NGO1024, encoding a protein belonging to the 2-nitropropane dioxygenase-like superfamily of proteins, was found to result in an inability of gonococci to grow anaerobically. Anaerobic growth of an NG1024 mutant was restored upon supplementation with unsaturated fatty acids (UFA), but not with the saturated fatty acid palmitate. Gonococcal fatty acid profiles confirmed that NGO1024 was involved in UFA synthesis anaerobically, but not aerobically, demonstrating that gonococci contain two distinct pathways for the production of UFAs, with a yet unidentified aerobic mechanism, and an anaerobic mechanism involving NGO1024. Expression of genes involved in classical anaerobic UFA synthesis, fabA, fabM and fabB, was toxic in gonococci and unable to complement a NGO1024 mutation, suggesting that the chemistry involved in gonococcal anaerobic UFA synthesis is distinct from that of the classical pathway. NGO1024 homologues, which we suggest naming UfaA, form a distinct lineage within the 2-nitropropane dioxygenase-like superfamily, and are found in many facultative and obligate anaerobes that produce UFAs but lack fabA, suggesting that UfaA is part of a widespread pathway involved in UFA synthesis.
Authors:
Vincent M Isabella; Virginia L Clark
Related Documents :
22497525 - Contributions of fasn and scd gene polymorphisms on fatty acid composition in muscle fr...
25194775 - Quantification and role of organic acids in cucumber root exudates in trichoderma harzi...
25441585 - Review on emu products for use as complementary and alternative medicine.
25300275 - Metabolomic biomarkers for obesity in humans: a short review.
11877335 - Multiple pathways for fluoroquinolone secretion by human intestinal epithelial (caco-2)...
7140775 - Chemical characterization of a rabbit leukocytic pyrogen.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-09-19
Journal Detail:
Title:  Molecular microbiology     Volume:  82     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-12     Completed Date:  2012-01-24     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  489-501     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, Box 672, 601 Elmwood Ave, Rochester, NY 14642, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Bacterial Proteins / genetics,  metabolism*
Dioxygenases / genetics,  metabolism*
Evolution, Molecular
Fatty Acids, Unsaturated / biosynthesis*
Hydro-Lyases / genetics,  metabolism*
Mutation
Neisseria gonorrhoeae / classification,  enzymology*,  genetics,  metabolism
Phylogeny
Grant Support
ID/Acronym/Agency:
R21 AI 080912/AI/NIAID NIH HHS; R21 AI080912-02/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Fatty Acids, Unsaturated; EC 1.13.11.-/Dioxygenases; EC 4.2.1.-/Hydro-Lyases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evolution of catabolic pathways and their regulatory systems in synthetic nitroaromatic compounds de...
Next Document:  Two oppositely oriented arrays of low-affinity recognition sites in oriC guide progressive binding o...