Document Detail

Identification of compounds that augment the lectin-sensitivity of Chinese Hamster Ovary cells.
MedLine Citation:
PMID:  22683639     Owner:  NLM     Status:  Publisher    
Glycosylation is now recognized as one of the most important modifications of eukaryotic proteins. In cancer biology, alterations in cell surface glycosylation have been exploited as valuable biomarkers, and the relationship of this modification to the metastatic characteristics of cancer cells has also been well-documented. Chemicals that can alter cell surface glycosylation patterns will therefore become attractive lead compounds for controlling the metastatic characteristics of cancer cells, one of the critical factors in their malignancy and prognosis of the disease. In this study, we established a system for screening compounds that have the potential to alter cell surface glycosylation by taking advantage of the susceptibility of cells toward various lectins. Through our screening of a chemical library, we were able to identify two compounds that augment the sensitivity of Chinese Hamster Ovary (CHO-K1) cells against the L4-PHA lectin. Surprisingly, these compounds did not result in alterations in cell surface glycan structures. Instead, they appeared to render the cells to be more sensitive to various lectins with distinct carbohydrate specificities. These compounds promise to be valuable, not only as tools for providing insights into the intracellular signalling of lectin-mediated growth arrest, but also as potential lead compounds for use as therapeutic, anti-cancer drugs.
Kumiko Ishii; Tamio Saito; Hiroyuki Osada; Naoyuki Taniguchi; Tadashi Suzuki
Related Documents :
396649 - G cell distribution in normal duodenal mucosa.
2880519 - The endocrine cells of the digestive tract. general concepts and historic perspective.
11401689 - Pathogenicity island-dependent activation of rho gtpases rac1 and cdc42 in helicobacter...
8774989 - A new model to study repair of gastric mucosa using primary cultured rabbit gastric epi...
18299709 - Lansoprazole novel effector sites revealed by autoradiography: relation to helicobacter...
14700549 - Helicobacter pylori-induced epithelial cell signalling in gastric carcinogenesis.
15948989 - Comparative analysis of the expression of erbin and erb-b2 in normal human skin and cut...
16636679 - Androgen receptor mutation (t877a) promotes prostate cancer cell growth and cell survival.
24515429 - Patterns of incorporation of d-galactose into cell wall polysaccharide of growing maize...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-5
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Glycometabolome Team, Systems Glycobiology Research Group, RIKEN Advanced Science Institute.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Physiological function, expression pattern, and transcriptional regulation of a Caenorhabditis elega...
Next Document:  Breath isoprene: muscle dystrophy patients support the concept of a pool of isoprene in the peripher...