Document Detail

Identification of a compound in Chamaecyparis taiwanensis inhibiting the ice-nucleating activity of Pseudomonas fluorescens KUIN-1.
MedLine Citation:
PMID:  11210129     Owner:  NLM     Status:  MEDLINE    
Inactivation of the ice-nucleating activity of Pseudomonas fluorescens KUIN-1 by compounds in the leaves from coniferous trees were investigated, and the inactivated material was identified. Intact cells of the strain KUIN-1 and the acetone or methanol extracts of leaves of various coniferous trees were allowed to react for 30 min at 18 degrees C. Antinucleation compounds were obtained from Chamaecyparis taiwanensis. When the acetone extract from the leaves of coniferous trees was added to the cell suspension (about 10(6) cells/ml) in 50 mM potassium phosphate buffer (pH 7.0), the ice nucleating temperature, T50, was significantly decreased (T50 < -5 degrees C). This inhibitor was isolated by using TLC, then identified as hinokitiol based on UV-VIS, IR, and mass spectral data. When intact cells of the strain KUIN-1 were incubated with hinokitiol, limonene, and alpha-pinene of the principal constituent of the leaves of coniferous trees in 50 mM potassium phosphate buffer (pH 7.0), the ice-nucleating activity decreased, but not in alpha-terpinene. Furthermore, the ice-nucleating activities from other ice-nucleating bacteria also decreased in the presence of hinokitiol. This inhibition was proportional to the concentration of hinokitinol. The pH and thermal stabilities of the ice-nucleating activity of the cells were changed by the addition of hinokitiol (10 mM).
H Kawahara; K Masuda; H Obata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  64     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-07     Completed Date:  2001-05-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2651-6     Citation Subset:  IM    
Department of Biotechnology, Faculty of Engineering, Kansai University, Suita, Osaka, Japan.
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MeSH Terms
Drug Evaluation, Preclinical / methods
Gas Chromatography-Mass Spectrometry
Plant Extracts / chemistry,  pharmacology
Pseudomonas fluorescens / drug effects*,  physiology*
Spectrophotometry, Ultraviolet
Terpenes / pharmacology
Trees / chemistry*
Tropolone / analogs & derivatives*,  isolation & purification,  pharmacology*
Reg. No./Substance:
0/Cyclohexenes; 0/Ice; 0/Monoterpenes; 0/Plant Extracts; 0/Terpenes; 138-86-3/limonene; 499-44-5/beta-thujaplicin; 533-75-5/Tropolone; 80-56-8/alpha-pinene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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