Document Detail

Identification and cloning of the type IIIa plasmid-encoded dihydrofolate reductase gene from trimethoprim-resistant gram-negative bacteria isolated in Britain.
MedLine Citation:
PMID:  2179557     Owner:  NLM     Status:  MEDLINE    
A clinical strain of Escherichia coli isolated in Nottinghamshire in 1980 was shown to harbour the type IIIa trimethoprim-resistant dihydrofolate reductase gene, previously identified on only one occasion, in New Zealand in 1979. The gene was identified by hybridisation with an 855-bp type III gene probe and its classification as a type IIIa dihydrofolate reductase was confirmed by detailed biochemical analysis of the enzyme product. The dihydrofolate reductase was identical in size and isoelectric point with the original type IIIa enzyme and shared similar inhibitory and kinetic profiles. The trimethoprim resistance gene was subsequently cloned and the type IIIa dihydrofolate reductase gene was localised to a 700-bp EcoRI-PstI fragment. This smaller fragment may prove to be a more specific DNA probe for the future identification of type IIIa dihydrofolate reductase genes.
C J Thomson; K J Towner; H K Young; S G Amyes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  31     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-23     Completed Date:  1990-04-23     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  213-8     Citation Subset:  IM    
Department of Bacteriology, Medical School, University of Edinburgh.
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MeSH Terms
Cloning, Molecular
DNA Probes
Escherichia coli / genetics*,  isolation & purification
Genes, Bacterial / genetics*
Plasmids / genetics*
Tetrahydrofolate Dehydrogenase / analysis,  genetics*,  metabolism
Transformation, Bacterial
Trimethoprim Resistance / genetics*
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/DNA Probes; EC Dehydrogenase

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