Document Detail


Identification and cloning of rat galectin-2: expression is predominantly in epithelial cells of the stomach.
MedLine Citation:
PMID:  9882446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A complementary DNA clone preferentially expressed in the gastrointestinal tract was obtained from a rat stomach library. The protein coded by the clone had a single carbohydrate recognition domain having conserved motifs for beta-galactoside binding and showed 67% amino acid identity with human galectin-2. The recombinant protein synthesized in Escherichia coli could bind to an asialofetuin column and was eluted with beta-galactopyranoside. From these observations, we named the protein rat galectin-2 coded by the cDNA. The rat galectin-2 was predominantly expressed in the epithelial cells of stomach. Thus this protein may form a mucin layer cross-linking with the beta-galactoside moiety of glycoproteins.
Authors:
T Oka; S Murakami; Y Arata; J Hirabayashi; K Kasai; Y Wada; M Futai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  361     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-11     Completed Date:  1999-02-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  195-201     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Institute of Scientific and Industrial Research, Osaka University, Osaka, Ibaraki, 567-0047, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB001075
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Epithelial Cells / metabolism*
Galectin 2
Gene Expression Regulation*
Hemagglutinins / biosynthesis,  chemistry,  genetics*
Humans
Intestines / metabolism
Lectins / biosynthesis,  chemistry,  genetics*
Molecular Sequence Data
Organ Specificity / genetics
Rats
Rats, Sprague-Dawley
Recombinant Proteins / chemistry,  isolation & purification
Sequence Homology, Amino Acid
Stomach / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Galectin 2; 0/Hemagglutinins; 0/Lectins; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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