| Identification of circulating fetal cell markers by microarray analysis. | |
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MedLine Citation:
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PMID: 22570279 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: Different fetal cell types have been found in the maternal blood during pregnancy in the past, but fetal cells are scarce, and the proportions of the different cell types are unclear. The objective of the present study was to identify specific fetal cell markers from fetal cells found in the maternal blood circulation at the end of the first trimester. METHOD: Twenty-three fetal cells were isolated from maternal blood by removing the red blood cells by lysis or combining this with removal of large proportions of maternal white blood cells by magnetic-activated cell sorting. Fetal cells identified by XY fluorescence in situ hybridization and confirmed by reverse-color fluorescence in situ hybridization were shot off microscope slides by laser capture microdissection. The expression pattern of a subset of expressed genes was compared between fetal cells and maternal blood cells using stem cell microarray analysis. RESULTS: Twenty-eight genes were identified as fetal cell marker candidates. CONCLUSION: Of the 28 fetal marker candidate genes, five coded for proteins, which are located on the outer surface of the cell membrane and not expressed in blood. The protein product of these five genes, MMP14, MCAM, KCNQ4, CLDN6, and F3, may be used as markers for fetal cell enrichment. © 2012 John Wiley & Sons, Ltd. |
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Authors:
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Marie Brinch; Lotte Hatt; Ripudaman Singh; Kristine Møller; Steffen Sommer; Niels Uldbjerg; Britta Christensen; Steen Kølvraa |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-9 |
Journal Detail:
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Title: Prenatal diagnosis Volume: - ISSN: 1097-0223 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8106540 Medline TA: Prenat Diagn Country: - |
Other Details:
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Languages: ENG Pagination: 1-10 Citation Subset: - |
Copyright Information:
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© 2012 John Wiley & Sons, Ltd. |
Affiliation:
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FCMB ApS, Vejle, Denmark. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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