Document Detail

Identification and chromosomal localisation by fluorescence in situ hybridisation of human gene of phosphoinositide-specific phospholipase C beta(1).
MedLine Citation:
PMID:  10760467     Owner:  NLM     Status:  MEDLINE    
Members of phosphoinositide-specific phospholipase C (PLC) families are central intermediary in signal transduction in response to the occupancy of receptors by many growth factors. Among PLC isoforms, the type beta(1) is of particular interest because of its reported nuclear localisation in addition to its presence at the plasma membrane. It has been previously shown that both the stimulation and the inhibition of the nuclear PLCbeta(1) under different stimuli implicate PLCbeta(1) as an important enzyme for mitogen-activated cell growth as well as for murine erythroleukaemia cell differentiation. The above findings hinting at a direct involvement of PLCbeta(1) in controlling the cell cycle in rodent cells, and the previously reported mapping of its gene in rat chromosome band 3q35-36, a region frequently rearranged in rat tumours induced by chemical carcinogenesis, prompted us to identify its human homologue. By screening a human foetal brain cDNA library with the rat PLCbeta(1) cDNA probe, we have identified a clone homologous to a sequence in gene bank called KIAA 0581, which encodes a large part of the human PLCbeta(1). By using this human cDNA in fluorescence in situ hybridisation on human metaphases, it has been possible to map human PLCbeta(1) on chromosome 20p12, confirming the synteny between rat chromosome 3 and human chromosome 20 and providing a novel locus of homology between bands q35-36 in rat and p12 in man. Since band 20p12 has been recently reported amplified and/or deleted in several solid tumours, the identification and chromosome mapping of human PLCbeta(1) could pave the way for further investigations on the role exerted both in normal human cells and in human tumours by PLCbeta(1), which has been shown to behave as a key signalling intermediate in the control of the cell cycle.
D Peruzzi; G Calabrese; I Faenza; L Manzoli; A Matteucci; F Gianfrancesco; A M Billi; L Stuppia; G Palka; L Cocco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1484     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-06-23     Completed Date:  2000-06-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  175-82     Citation Subset:  IM    
Cellular Signalling Laboratory, Institute of Anatomy at the University of Bologna, Via Irnerio 48, I-40126, Bologna, Italy.
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MeSH Terms
Base Sequence
Blotting, Northern
Brain / enzymology
Chromosome Mapping
Gene Library
In Situ Hybridization, Fluorescence
Isoenzymes / chemistry,  genetics*
Molecular Sequence Data
Phospholipase C beta
Polymerase Chain Reaction
Type C Phospholipases / chemistry,  genetics*
Reg. No./Substance:
0/Isoenzymes; EC 3.1.4.-/Type C Phospholipases; EC protein, human; EC C beta; EC protein, rat

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