Document Detail

Identification of a chromosomal Shigella flexneri multi-antibiotic resistance locus which shares sequence and organizational similarity with the resistance region of the plasmid NR1.
MedLine Citation:
PMID:  9200219     Owner:  NLM     Status:  MEDLINE    
The ampicillin resistance gene from Shigella flexneri 2a strain YSH6000 was cloned and shown by Southern hybridization analysis to be closely linked to the previously cloned streptomycin, chloramphenicol, and tetracycline resistance determinants, which are borne on a chromosomally integrated 99-kb element. Analysis of this chromosomal multi-antibiotic resistance locus revealed that it had a high level of sequence and organizational similarity to an equivalent region of the Shigella R-plasmid, NR1. However, the chromosomal locus exhibited several differences, including the presence of two stretches of sequence derived from IS elements, the precise insertion of a beta-lactamase encoding oxal cassette into the Tn21-borne integron In2, a possible 17.5-kb deletion, and the loss or inactivation of the mercury resistance determinant. Based on these data, it is proposed that the chromosomal locus arose following integration of an NR1-like plasmid.
K Rajakumar; D Bulach; J Davies; L Ambrose; C Sasakawa; B Adler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plasmid     Volume:  37     ISSN:  0147-619X     ISO Abbreviation:  Plasmid     Publication Date:  1997  
Date Detail:
Created Date:  1997-08-06     Completed Date:  1997-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802221     Medline TA:  Plasmid     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  159-68     Citation Subset:  IM    
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J01830;  J02967;  M10791;  M62822;  U81136;  U81137;  U81138;  U81139;  U81140;  U81141;  U81142;  V00609;  X04891;  X05952;  X12870
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MeSH Terms
Ampicillin / pharmacology
Blotting, Southern
Chloramphenicol / pharmacology
Chromosomes, Bacterial / genetics*
Cloning, Molecular
DNA, Bacterial / analysis
Drug Resistance, Microbial / genetics*
Drug Resistance, Multiple / genetics*
Genes, MDR / genetics*
Linkage (Genetics)
Mercuric Chloride / pharmacology
Microbial Sensitivity Tests
Molecular Sequence Data
R Factors / genetics*
Sequence Analysis, DNA
Shigella flexneri / drug effects,  genetics*
Streptomycin / pharmacology
Sulfathiazoles / pharmacology
Tetracycline / pharmacology
Reg. No./Substance:
0/DNA, Bacterial; 0/Sulfathiazoles; 56-75-7/Chloramphenicol; 57-92-1/Streptomycin; 60-54-8/Tetracycline; 69-53-4/Ampicillin; 72-14-0/sulfathiazole; 7487-94-7/Mercuric Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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