| Identification of cholesterol crystals in plaques of atherosclerotic mice using hyperspectral CARS imaging. | |
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MedLine Citation:
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PMID: 21949051 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The accumulation of lipids, including cholesterol, in the arterial wall plays a key role in the pathogenesis of atherosclerosis. Although several advances have been made in the detection and imaging of these lipid structures in plaque lesions, their morphology and composition have yet to be fully elucidated, particularly in different animal models of disease. To address this issue, we analyzed lipid morphology and composition in the atherosclerotic plaques of two animal models of disease - the low-density lipoprotein receptor-deficient mouse and the ApoE lipoprotein-deficient mouse - utilizing hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy in combination with principal component analysis (PCA). Hyperspectral CARS imaging revealed lipid-rich macrophage cells and condensed needle-shaped and plate-shaped lipid crystal structures in both mice. Spectral analysis with PCA and comparison to spectra of pure cholesterol and cholesteryl ester derivatives further revealed these lipid structures to be pure cholesterol crystals, which were predominantly observed in the ApoE-deficient mouse model. These results illustrate the ability of hyperspectral CARS imaging in combination with multivariate analysis to characterize atherosclerotic lipid morphology and composition with chemical specificity, and consequently, provide new insight into the formation of cholesterol crystal structures in atherosclerotic plaque lesions. |
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Authors:
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Ryan S Lim; Jeffrey L Suhalim; Shinobu Miyazaki-Anzai; Makoto Miyazaki; Moshe Levi; Eric O Potma; Bruce J Tromberg |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-23 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of California, Irvine, United States; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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