Document Detail


Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae.
MedLine Citation:
PMID:  21317321     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tfoX (also called sxy) gene product is the central regulator of DNA uptake in the naturally competent bacteria Haemophilus influenzae and Vibrio cholerae. However, the mechanisms regulating tfoX gene expression in both organisms are poorly understood. Our previous studies revealed that in V. cholerae, chitin disaccharide (GlcNAc)₂ is needed to activate the transcription and translation of V. cholerae tfoX (tfoX(VC)) to induce natural competence. In this study, we screened a multicopy library of V. cholerae DNA fragments necessary for translational regulation of tfoX(VC). A clone carrying the VC2078-VC2079 intergenic region, designated tfoR, increased the expression of a tfoX(VC)::lacZ translational fusion constructed in Escherichia coli. Using a tfoX(VC)::lacZ reporter system in V. cholerae, we confirmed that tfoR positively regulated tfoX(VC) expression at the translational level. Deletion of tfoR abolished competence for exogenous DNA even when (GlcNAc)₂ was provided. The introduction of a plasmid clone carrying the tfoR(+) gene into the tfoR deletion mutant complemented the competence deficiency. We also found that the tfoR gene encodes a 102-nucleotide small RNA (sRNA), which was transcriptionally activated in the presence of (GlcNAc)₂. Finally, we showed that this sRNA activated translation from tfoX(VC) mRNA in a highly purified in vitro translation system. Taking these results together, we propose that in the presence of (GlcNAc)₂, TfoR sRNA is expressed to activate the translation of tfoX(VC), which leads to the induction of natural competence.
Authors:
Shouji Yamamoto; Hidemasa Izumiya; Jiro Mitobe; Masatomo Morita; Eiji Arakawa; Makoto Ohnishi; Haruo Watanabe
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-11
Journal Detail:
Title:  Journal of bacteriology     Volume:  193     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-08     Completed Date:  2011-06-06     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1953-65     Citation Subset:  IM    
Affiliation:
Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan. yshouji@nih.go.jp
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB598400
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MeSH Terms
Descriptor/Qualifier:
Artificial Gene Fusion
Base Sequence
Chitin / metabolism*
DNA, Bacterial / chemistry,  genetics
Escherichia coli / genetics
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation, Bacterial*
Gene Library
Genes, Reporter
Genetic Complementation Test
Genetic Testing
Molecular Sequence Data
Protein Biosynthesis*
RNA, Small Interfering / metabolism*
Sequence Analysis, DNA
Transformation, Bacterial*
Vibrio cholerae / genetics*,  metabolism*
beta-Galactosidase / genetics,  metabolism
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Small Interfering; 1398-61-4/Chitin; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

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