Document Detail

Identification and characterization of two novel splice forms of GRIP1 in the rat brain.
MedLine Citation:
PMID:  16539648     Owner:  NLM     Status:  MEDLINE    
We cloned two novel alternatively-spliced mRNA isoforms of glutamate receptor interacting protein 1 (GRIP1) which we named GRIP1d and GRIP1e 4-7. GRIP1d is a 135 kDa, 7-PDZ-domain variant of GRIP1, containing the 12 amino acid C-terminus originally described for the 4-PDZ-domain GRIP1c 4-7. GRIP1e 4-7 is a 75 kDa 4-PDZ-domain variant of GRIP1, containing the 12 amino acid C-terminus originally described for the 7-PDZ-domain GRIP1a/b. Northern blots indicated that GRIP1d mRNA is 5.1 kb long and abundant in brain. An antibody to the C-terminus of the 75 kDa GRIP1c 4-7 also recognized an abundant 135 kDa protein, consistent with the predicted size of GRIP1d. Similarly, an antibody to the C-terminus of the 135 kDa GRIP1a/b also recognized a low abundance 75 kDa protein, consistent with the predicted size of GRIP1e 4-7. Immunocytochemistry of hippocampal cultures and intact brain using these antibodies showed that (i) these isoforms are present in both GABAergic and glutamatergic synapses, and (ii) the isoforms co-localize in individual synapses. While GRIP1a/b isoforms are abundant in interneurons and highly concentrated in GABAergic presynaptic terminals, the isoforms recognized by the antibody to the C-terminus common to GRIP1c 4-7 and GRIP1d are much less abundant in interneurons and preferentially concentrate at the postsynaptic complex.
Erik I Charych; Rongwen Li; David R Serwanski; Xuejing Li; Celia P Miralles; Noelia Pinal; Angel L De Blas
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2006-03-15
Journal Detail:
Title:  Journal of neurochemistry     Volume:  97     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-08     Completed Date:  2006-06-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  884-98     Citation Subset:  IM    
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269-3156, USA.
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MeSH Terms
Alternative Splicing / genetics*
Amino Acid Sequence
Blotting, Northern / methods
Brain / cytology,  metabolism*,  ultrastructure
Carrier Proteins / genetics*,  metabolism*
Cells, Cultured
Cloning, Molecular / methods
Embryo, Mammalian
Glutamate Decarboxylase / metabolism
Hippocampus / cytology
Immunohistochemistry / methods
Intracellular Signaling Peptides and Proteins / metabolism
Membrane Proteins / metabolism
Microscopy, Immunoelectron / methods
Molecular Weight
Nerve Tissue Proteins / genetics*,  metabolism*
Neurons / cytology,  metabolism
Oligonucleotide Array Sequence Analysis / methods
Protein Isoforms / genetics,  metabolism
Rats, Sprague-Dawley
Sequence Alignment / methods
Synapses / metabolism,  ultrastructure
Vesicular Glutamate Transport Protein 1 / metabolism
Grant Support
Reg. No./Substance:
0/Carrier Proteins; 0/Dlgh4 protein, rat; 0/Grip1 protein, rat; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Protein Isoforms; 0/Vesicular Glutamate Transport Protein 1; EC Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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