| Identification and characterization of two novel lysozymes from Rhodnius prolixus, a vector of Chagas disease. | |
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MedLine Citation:
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PMID: 18258253 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lysozymes have been described in invertebrates as digestive or immune molecules. We report here the characterization of two novel c-type lysozymes, RpLys-A (EU250274) and RpLys-B (EU250275), isolated from the fat body and digestive tract of immune stimulated Rhodnius prolixus, a major vector of Chagas disease. Transcriptional profiles indicate that the temporal and spatial expression patterns of these two peptides are very different. RpLys-A is expressed predominantly in the midgut after ingestion of Trypanosoma cruzi in a bloodmeal, or after injection of bacteria into the hemocoel. RpLys-B is expressed primarily in the fat body after bacterial injection. Phylogenetic alignments indicate that RpLys-A aligns best with molecules from other hemipterans whose major expression is found in the intestinal tract whereas RpLys-B aligns best with mosquito and tick molecules whose expression is found principally in hemocytes and fat body and whose role has been described as immune-related. These data suggest a differential compartmentalized role of two closely related molecules; one for immunity in the hemocoel and the other for digestion in the midgut. |
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Authors:
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Raul J Ursic-Bedoya; Hamed Nazzari; Dawn Cooper; Omar Triana; Marta Wolff; Carl Lowenberger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-31 |
Journal Detail:
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Title: Journal of insect physiology Volume: 54 ISSN: 0022-1910 ISO Abbreviation: J. Insect Physiol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-19 Completed Date: 2008-07-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985080R Medline TA: J Insect Physiol Country: England |
Other Details:
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Languages: eng Pagination: 593-603 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Simon Fraser University, 8888 University Dr., Burnaby, BC, Canada V5A 1S6. rursicbe@sfu.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Chagas Disease / transmission Escherichia coli / immunology Feeding Behavior / physiology Host-Parasite Interactions / physiology Humans Micrococcus luteus / immunology Molecular Sequence Data Muramidase / genetics, metabolism* Phylogeny Promoter Regions, Genetic Rhodnius / enzymology*, parasitology, physiology Sequence Alignment Sequence Analysis, DNA Time Factors Trypanosoma cruzi / physiology |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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