Document Detail


Identification and characterization of two bile acid coenzyme A transferases from Clostridium scindens, a bile acid 7α-dehydroxylating intestinal bacterium.
MedLine Citation:
PMID:  22021638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human bile acid pool composition is composed of both primary bile acids (cholic acid and chenodeoxycholic acid) and secondary bile acids (deoxycholic acid and lithocholic acid). Secondary bile acids are formed by the 7α-dehydroxylation of primary bile acids carried out by intestinal anaerobic bacteria. We have previously described a multistep biochemical pathway in Clostridium scindens that is responsible for bile acid 7α-dehydroxylation. We have identified a large (12 kb) bile acid inducible (bai) operon in this bacterium that encodes eight genes involved in bile acid 7α-dehydroxylation. However, the function of the baiF gene product in this operon has not been elucidated. In the current study, we cloned and expressed the baiF gene in E. coli and discovered it has bile acid CoA transferase activity. In addition, we discovered a second bai operon encoding three genes. The baiK gene in this operon was expressed in E. coli and found to encode a second bile acid CoA transferase. Both bile acid CoA transferases were determined to be members of the type III family by amino acid sequence comparisons. Both bile acid CoA transferases had broad substrate specificity, except the baiK gene product, which failed to use lithocholyl-CoA as a CoA donor. Primary bile acids are ligated to CoA via an ATP-dependent mechanism during the initial steps of 7α-dehydroxylation. The bile acid CoA transferases conserve the thioester bond energy, saving the cell ATP molecules during bile acid 7α-dehydroxylation. ATP-dependent CoA ligation is likely quickly supplanted by ATP-independent CoA transfer.
Authors:
Jason M Ridlon; Phillip B Hylemon
Related Documents :
15913768 - Enhanced effects of lithocholic acid incorporation into liquid-crystalline biopolymer p...
22724388 - Ornithine lipids and their structural modifications: from a to e and beyond.
2384688 - Effects of aging and xerosis on the amino acid composition of human skin.
2889138 - Identification and characterization of thy-1 homologues from bovine thymocytes.
23283728 - Mass spectrometric distinction of in-source and in-solution pyroglutamate and succinimi...
10078938 - Constitutive expression of 8-lipoxygenase in papillomas and clastogenic effects of lipo...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-20
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-16     Completed Date:  2012-04-09     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  66-76     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU675330
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bile Acids and Salts / metabolism
Cloning, Molecular
Clostridium / enzymology*
Coenzyme A-Transferases / chemistry,  isolation & purification,  metabolism*
Escherichia coli / enzymology
Hydroxysteroid Dehydrogenases / metabolism
Intestines / microbiology
Molecular Sequence Data
Multigene Family
Substrate Specificity
Grant Support
ID/Acronym/Agency:
2T32DK-007150-35/DK/NIDDK NIH HHS; P01DK-38030/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; EC 1.1.-/Hydroxysteroid Dehydrogenases; EC 1.1.1.159/7 alpha-hydroxysteroid dehydrogenase; EC 2.8.3.-/Coenzyme A-Transferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Structural characterization of saturated branched chain fatty acid methyl esters by collisional diss...
Next Document:  Relations among Socioeconomic Status Indicators and Health for African-Americans and Whites.