Document Detail


Identification and characterization of thiamin repressible acid phosphatase in yeast.
MedLine Citation:
PMID:  3536917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified a genetic locus, pho4, in Schizosaccharomyces pombe which encodes a minor expressed cell surface acid phosphatase that is repressed by low concentrations (0.5 microM) of thiamin. The enzyme was purified from a strain that overproduces the enzyme. It is an Asn-linked glycoprotein. Removal of the carbohydrates by endoglycosidase H does not abolish enzymatic activity. The molecular mass of deglycosylated and unglycosylated enzyme that accumulates in membranes when cells are grown in the presence of tunicamycin is 56 kDa as determined by sodium dodecyl sulfate-gel electrophoresis. Thiamin regulation, at least in part, operates by reducing the level of pho4-mRNA. Pho4 is not genetically linked to the phosphate repressible acid phosphatase gene pho1. Phosphate and thiamin repressible acid phosphatase differ in their substrate specificity. Their protein moieties are immunologically related. Pho4 and pho1 are the only genes in S. pombe that express cell surface acid phosphatases being enzymatically active with nitrophenyl phosphate as substrate. S. pombe is not unique in having a thiamin repressible acid phosphatase. In Saccharomyces cerevisiae this enzyme is encoded by PHO3.
Authors:
M E Schweingruber; R Fluri; K Maundrell; A M Schweingruber; E Dumermuth
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  261     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-01-07     Completed Date:  1987-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15877-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / analysis,  genetics,  isolation & purification*
Enzyme Repression
Glycosylation
Phosphates / pharmacology
RNA, Messenger / analysis
Saccharomyces cerevisiae / enzymology
Saccharomycetales / enzymology*
Schizosaccharomyces / enzymology*
Substrate Specificity
Thiamine / pharmacology*
Tunicamycin / pharmacology
Chemical
Reg. No./Substance:
0/Phosphates; 0/RNA, Messenger; 11089-65-9/Tunicamycin; 59-43-8/Thiamine; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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