Document Detail

Identification and characterization of rodent ABCA1 in isolated type II pneumocytes.
MedLine Citation:
PMID:  12909583     Owner:  NLM     Status:  MEDLINE    
ATP-binding cassette transporter A1 (ABCA1) promotes transfer of cholesterol and phospholipid from cells to lipid-free serum apolipoproteins. ABCA1 mRNA and protein expression in primary cultures of rodent type II cells was sensitive to upregulation with 5 microM 9-cis-retinoic acid (9cRA) and 6.2 microM 22-hydroxycholesterol (22-OH). The increase in ABCA1 protein levels was time dependent and was maximal after 16 h of exposure to 9cRA + 22-OH. Inducible ABCA1 was also found in transformed cell lines of lung origin: WI38/VA13, A549, and NIH-H441 cells. Stimulation of ABCA1 in rat type II cells by 9cRA + 22-OH resulted in a four- or fivefold enhancement of efflux of radioactive phospholipid or cholesterol, respectively, from the pneumocytes to apolipoprotein AI (apo AI), whereas cAMP (0.3 mM) had no effect. ABCA1-mediated lipid efflux to apo AI was independent of the surfactant secretion pathway, inasmuch as upregulation of ABCA1 resulted in a reduction of secretagogue-stimulated surfactant phospholipid release. These studies demonstrate the presence of functional ABCA1 in type II cells from the lung.
Anna E Bortnick; Elda Favari; Jian-Qin Tao; Omar L Francone; Muredach Reilly; Yuzhen Zhang; George H Rothblat; Sandra R Bates
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-08-08
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  285     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-08     Completed Date:  2003-10-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L869-78     Citation Subset:  IM    
Institute for Environmental Medicine, University of Pennsylvania, 1 John Morgan Bldg., 36th and Hamilton Walk, Philadelphia, PA 19104-6068, USA.
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MeSH Terms
ATP-Binding Cassette Transporters / genetics,  metabolism,  physiology*
Apolipoprotein A-I / pharmacology
Cell Line, Transformed
Cholesterol / metabolism
Hydroxycholesterols / pharmacology
Lung / cytology,  metabolism*
Phospholipids / metabolism
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Time Factors
Tretinoin / pharmacology
Grant Support
Reg. No./Substance:
0/ATP binding cassette transporter 1; 0/ATP-Binding Cassette Transporters; 0/Apolipoprotein A-I; 0/Hydroxycholesterols; 0/Phospholipids; 0/RNA, Messenger; 17711-16-9/22-hydroxycholesterol; 302-79-4/Tretinoin; 5300-03-8/alitretinoin; 57-88-5/Cholesterol

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