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Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells.
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MedLine Citation:
PMID:  3097239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We show here that human peripheral blood NK cells contain a pore-forming protein (PFP) with an Mr of 70,000-72,000 that assembles structural lesions (with an average internal diameter of 150-170 A) and forms functional channels. The PFP was isolated by affinity chromatography from human NK cells, using a specific anti-C9 antiserum as the immunoadsorbent. The NK cells were isolated from PBL by positive or negative selection by indirect rosetting using a panel of monoclonal antibodies directed against different NK and T cell surface antigens. PFP was identified in NK cells freshly isolated and isolated from cultured PBL, both stimulated with interleukin 2, but not in NK cell-depleted lymphocytes. In planar bilayers, the channels formed by the NK cell-derived PFP are highly voltage resistant, with most channels persisting in the open state once they have inserted into the bilayer. The unit conductances of these channels range 0.3-1 nS in 0.1 M NaCl. The channels show poor selectivity for monovalent and divalent ions. The PFP is also released from human NK cells stimulated with the calcium ionophore A23187, suggesting that this protein, like the one produced by murine CTL lines, may be similarly secreted during cell-mediated killing. Its identification in primary human NK cell cultures indicates that this protein may play an active role in NK cell-mediated killing.
Authors:
C C Liu; B Perussia; Z A Cohn; J D Young
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  164     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-01-08     Completed Date:  1987-01-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2061-76     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Calcimycin / pharmacology
Cell Line
Chromatography, Affinity
Cytotoxicity, Immunologic
Electrophoresis, Polyacrylamide Gel
Humans
Immunosorbent Techniques
Kidney Tubules / ultrastructure
Killer Cells, Natural / analysis*,  immunology
Membrane Glycoproteins*
Membrane Proteins / analysis*
Molecular Weight
Perforin
Phenotype
Pore Forming Cytotoxic Proteins
Rosette Formation
Grant Support
ID/Acronym/Agency:
AI-07012/AI/NIAID NIH HHS; CA-30198/CA/NCI NIH HHS; CA-37155/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Pore Forming Cytotoxic Proteins; 126465-35-8/Perforin; 52665-69-7/Calcimycin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 12 Year: 1986
Volume: 164 Issue: 6
First Page: 2061 Last Page: 2076
ID: 2188475
Publisher Id: 87059655
PubMed Id: 3097239

Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells


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