| Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells. | |
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MedLine Citation:
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PMID: 3097239 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We show here that human peripheral blood NK cells contain a pore-forming protein (PFP) with an Mr of 70,000-72,000 that assembles structural lesions (with an average internal diameter of 150-170 A) and forms functional channels. The PFP was isolated by affinity chromatography from human NK cells, using a specific anti-C9 antiserum as the immunoadsorbent. The NK cells were isolated from PBL by positive or negative selection by indirect rosetting using a panel of monoclonal antibodies directed against different NK and T cell surface antigens. PFP was identified in NK cells freshly isolated and isolated from cultured PBL, both stimulated with interleukin 2, but not in NK cell-depleted lymphocytes. In planar bilayers, the channels formed by the NK cell-derived PFP are highly voltage resistant, with most channels persisting in the open state once they have inserted into the bilayer. The unit conductances of these channels range 0.3-1 nS in 0.1 M NaCl. The channels show poor selectivity for monovalent and divalent ions. The PFP is also released from human NK cells stimulated with the calcium ionophore A23187, suggesting that this protein, like the one produced by murine CTL lines, may be similarly secreted during cell-mediated killing. Its identification in primary human NK cell cultures indicates that this protein may play an active role in NK cell-mediated killing. |
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Authors:
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C C Liu; B Perussia; Z A Cohn; J D Young |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of experimental medicine Volume: 164 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1986 Dec |
Date Detail:
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Created Date: 1987-01-08 Completed Date: 1987-01-08 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2061-76 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcimycin
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pharmacology Cell Line Chromatography, Affinity Cytotoxicity, Immunologic Electrophoresis, Polyacrylamide Gel Humans Immunosorbent Techniques Kidney Tubules / ultrastructure Killer Cells, Natural / analysis*, immunology Membrane Glycoproteins* Membrane Proteins / analysis* Molecular Weight Perforin Phenotype Pore Forming Cytotoxic Proteins Rosette Formation |
| Grant Support | |
ID/Acronym/Agency:
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AI-07012/AI/NIAID NIH HHS; CA-30198/CA/NCI NIH HHS; CA-37155/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Pore Forming Cytotoxic Proteins; 126465-35-8/Perforin; 52665-69-7/Calcimycin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 12 Year: 1986 Volume: 164 Issue: 6 First Page: 2061 Last Page: 2076 ID: 2188475 Publisher Id: 87059655 PubMed Id: 3097239 |
| Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells | |
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