Document Detail

Identification and characterization of a polysaccharide-containing antigen from Schistosoma mansoni eggs which cross-reacts with the surface of schistosomula.
MedLine Citation:
PMID:  2438628     Owner:  NLM     Status:  MEDLINE    
Antisera were produced by immunizing rabbits with either a trichloroacetic acid-soluble fraction, or a high molecular weight (Mr) fraction of Schistosoma mansoni SEA (a saline-soluble fraction of homogenized egg). Both of these sera reacted monospecifically in immunoelectrophoresis against unfractionated SEA, recognizing a cathodally migrating antigen. This antigen had been identified previously as being responded to by S. mansoni-infected mouse sera, and has been designated K3 (Kappa 3). The rabbit antisera were used to partially characterize antigen K3 as having Mr in the range greater than 750-70 K, and being resistant to boiling, resistant to the action of proteases, but sensitive to periodate. It partially binds to Concanavalin A. In addition to SEA, the antigen was present in homogenized cercariae and schistosomula, but not adult worms, and it was also present in detergent extracts of intact cercariae and schistosomula. Using an antibody-dependent cell adherence assay, anti-K3 serum was found to react with the surface of live cercariae and with the surface of schistosomula recovered from the skin of mice infected up to 48 h previously. Anti-K3 serum also reacted with the surface of S. bovis, S. haematobium and to a lesser extent S. japonicum schistosomula.
D W Dunne; Q D Bickle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parasitology     Volume:  94 ( Pt 2)     ISSN:  0031-1820     ISO Abbreviation:  Parasitology     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-07-14     Completed Date:  1987-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401121     Medline TA:  Parasitology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  255-68     Citation Subset:  IM    
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MeSH Terms
Antigens, Helminth / immunology*
Antigens, Surface / immunology
Cross Reactions
Ovum / immunology
Polysaccharides / analysis,  immunology
Schistosoma mansoni / immunology*
Species Specificity
Reg. No./Substance:
0/Antigens, Helminth; 0/Antigens, Surface; 0/Epitopes; 0/Polysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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