Document Detail


Identification and characterization of pathogenic Yersinia enterocolitica isolates by PCR and pulsed-field gel electrophoresis.
MedLine Citation:
PMID:  16000776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Approximately 550 to 600 yersiniosis patients are reported annually in Sweden. Although pigs are thought to be the main reservoir of food-borne pathogenic Yersinia enterocolitica, the role of pork meat as a vehicle for transmission to humans is still unclear. Pork meat collected from refrigerators and local shops frequented by yersiniosis patients (n=48) were examined for the presence of pathogenic Yersinia spp. A combined culture and PCR method was used for detection, and a multiplex PCR was developed and evaluated as a tool for efficient identification of pathogenic food and patient isolates. The results obtained with the multiplex PCR were compared to phenotypic test results and confirmed by pulsed-field gel electrophoresis (PFGE). In all, 118 pork products (91 raw and 27 ready-to-eat) were collected. Pathogenic Yersinia spp. were detected by PCR in 10% (9 of 91) of the raw pork samples (loin of pork, fillet of pork, pork chop, ham, and minced meat) but in none of the ready-to-eat products. Isolates of Y. enterocolitica bioserotype 4/O:3 were recovered from six of the PCR-positive raw pork samples; all harbored the virulence plasmid. All isolates were recovered from food collected in shops and, thus, none were from the patients' home. When subjected to PFGE, the six isolates displayed four different NotI profiles. The same four NotI profiles were also present among isolates recovered from the yersiniosis patients. The application of a multiplex PCR was shown to be an efficient tool for identification of pathogenic Y. enterocolitica isolates in naturally contaminated raw pork.
Authors:
S Thisted Lambertz; M-L Danielsson-Tham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  71     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-07     Completed Date:  2005-09-13     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3674-81     Citation Subset:  IM    
Affiliation:
National Food Administration, Research and Development Department, PO Box 622, SE-751 26 Uppsala, Sweden. sula@slv.se
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Animals
Bacterial Proteins / genetics
Child
Child, Preschool
DNA, Bacterial / analysis
Electrophoresis, Gel, Pulsed-Field / methods*
Female
Genotype
Humans
Infant
Male
Meat / microbiology
Meat Products / microbiology
Middle Aged
Phenotype
Polymerase Chain Reaction / methods*
Swine
Virulence / genetics
Yersinia Infections / microbiology
Yersinia enterocolitica / classification*,  genetics,  isolation & purification,  pathogenicity*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial
Comments/Corrections

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