Document Detail


Identification and characterization of oxylipid-protein and Peptide conjugates by mass spectrometry.
MedLine Citation:
PMID:  23045005     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The modification of proteins by reactive products of lipid peroxidation is associated with a large number of diseases and biological aging; thus, methods that enable the characterization of oxylipid-protein and/or peptide conjugates are highly in demand. This unit outlines a chemical labeling approach to identifying and characterizing proteins modified by lipid peroxidation products. It also outlines two approaches for mass spectrometry-based identification and detailed characterization of oxylipid conjugates. The first combines chemical labeling of oxylipid-protein conjugates using an aldehyde-specific biotinylation reagent, electrophoretic separation, and mass spectrometry-based identification of the biotinylated proteins. In the second approach, protein extracts are treated with the aldehyde-specific reagent, proteolyzed using trypsin, and the biotinylated peptides are enriched using immobilized monomeric avidin. The enriched peptide fractions are submitted to tandem mass spectrometry for determining the peptide sequence information, site of the modification, and chemical nature of the oxylipid. Curr. Protoc. Toxicol. 35:17.9.1-17.9.19. © 2008 by John Wiley & Sons, Inc.
Authors:
Woon-Gye Chung; Claudia S Maier
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]     Volume:  Chapter 17     ISSN:  1934-9262     ISO Abbreviation:  Curr Protoc Toxicol     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2012-10-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9816330     Medline TA:  Curr Protoc Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit17.9     Citation Subset:  IM    
Affiliation:
Oregon State University, Department of Chemistry, Corvallis, Oregon.
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