| Identification and characterization of nuclear matrix-attachment regions in the human serpin gene cluster at 14q32.1. | |
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MedLine Citation:
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PMID: 10481016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matrix-attachment regions (MARs) are DNA elements that are defined by their abilities to bind to isolated nuclear matrices in vitro. The DNA sequences of different matrix-binding elements vary widely. The locations of some MARs at the ends of chromatin loops suggest that they may represent boundaries of individual chromatin domains. As such, MARs may play important roles in regulating transcription and chromatin structure. As a first step towards assessing the roles of MARs in these processes, we assayed DNA sequences from the human serine protease inhibitor (serpin) gene cluster at 14q32.1 for matrix-binding activity in vitro. This approximately 150 kb region contains the cell-specific genes encoding alpha1-anti-trypsin (alpha1AT) and corticosteroid-binding globulin (CBG), as well as an antitrypsin-related sequence termed ATR. A DNase I-hypersensitive site (DHS) map of the locus has recently been described. We report here that the alpha1AT-ATR-CBG region contains five distinct MARs. There is a strong matrix-binding element approximately 16 kb upstream of alpha1AT; three MARs are between ATR and CBG and one MAR is within the CBG gene itself. These MARs were matrix-associated in all cell types examined. DNA sequencing indicated that the serpin MARs contained predominantly repetitive DNA, although the types of DNA repeats differed among the MARs. |
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Authors:
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P Rollini; S J Namciu; M D Marsden; R E Fournier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 27 ISSN: 1362-4962 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-12-17 Completed Date: 1999-12-17 Revised Date: 2008-11-20 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3779-91 Citation Subset: IM |
Affiliation:
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Division of Basic Sciences, A2-025, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, PO Box 19024, Seattle, WA 98109-1024, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF156542; AF156543; AF156544; AF156545 |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Binding Sites Chromosomes, Human, Pair 14* Cosmids DNA, Complementary Hela Cells Humans Molecular Sequence Data Multigene Family* Nuclear Matrix / metabolism* Research Design Sequence Analysis, DNA Serpins / genetics* Transcortin / genetics* Tumor Cells, Cultured alpha 1-Antitrypsin / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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DK09188/DK/NIDDK NIH HHS; GM26449/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Serpins; 0/alpha 1-Antitrypsin; 9010-38-2/Transcortin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 1 Month: 10 Year: 1999 Volume: 27 Issue: 19 First Page: 3779 Last Page: 3791 ID: 148640 PubMed Id: 10481016 Publisher Item Identifier: gkc577 |
| Identification and characterization of nuclear matrix-attachment regions in the human serpin gene cluster at 14q32.1. | |
| P Rollini | |
| S J Namciu | |
| M D Marsden | |
| R E Fournier | |
| Division of Basic Sciences, A2-025, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, PO Box 19024, Seattle, WA 98109-1024, USA. |
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