Document Detail


Identification and characterization of a novel transcriptional target of RUNX1/AML1 at the onset of hematopoietic development.
MedLine Citation:
PMID:  21498670     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the critical requirement for the transcription factor RUNX1/AML1 at the onset of hematopoietic development is well established, little is known about its transcriptional targets at this pivotal stage of blood development. Using microarrays, we identified the uncharacterized gene AI467606 as a gene whose expression level is dramatically reduced in the absence of RUNX1. We further demonstrated by chromatin immunoprecipitation and promoter assay a direct regulation of its transcription by RUNX1. Using a bacterial artificial chromosome transgenic approach, we established that AI467606 is expressed during the development of the hematopoietic system in vivo and in vitro and that its expression is detected within the CD41(+) population and marks definitive hematopoietic potential. Similarly, in the adult mouse, all hematopoietic cell lineages, except mature erythrocytes, express AI467606. Taken together, these findings indicate that AI467606 is a novel transcriptional target of RUNX1/AML1 at the onset of hematopoietic development that is extensively expressed within the hematopoietic system.
Authors:
Cristina Ferreras; Christophe Lancrin; Michael Lie-A-Ling; Valerie Kouskoff; Georges Lacaud
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-15
Journal Detail:
Title:  Blood     Volume:  118     ISSN:  1528-0020     ISO Abbreviation:  Blood     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-22     Completed Date:  2011-10-07     Revised Date:  2014-10-21    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  594-7     Citation Subset:  AIM; IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AI467606
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Bone Marrow / embryology,  physiology
Cell Lineage / physiology
Cells, Cultured
Chromosomes, Artificial, Bacterial / genetics
Core Binding Factor Alpha 2 Subunit / genetics,  metabolism*
Gene Expression Regulation, Developmental / physiology
Hematopoiesis / physiology*
Hematopoietic Stem Cells / cytology,  metabolism,  physiology
Mice
Molecular Sequence Data
Platelet Membrane Glycoprotein IIb / metabolism
Proteins / genetics*,  metabolism*
Transcription, Genetic / physiology*
Grant Support
ID/Acronym/Agency:
12486//Cancer Research UK; 12487//Cancer Research UK; //Biotechnology and Biological Sciences Research Council; //Cancer Research UK
Chemical
Reg. No./Substance:
0/AI467606 protein, mouse; 0/Core Binding Factor Alpha 2 Subunit; 0/Platelet Membrane Glycoprotein IIb; 0/Proteins; 0/Runx1 protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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