Document Detail


Identification and characterization of a novel human collectin CL-K1.
MedLine Citation:
PMID:  17179669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Collectins are a family of C-type lectins with two characteristic structures, collagen like domains and carbohydrate recognition domains. They recognize carbohydrate antigens on microorganisms and act as host-defense. Here we report the cloning and characterization of a novel collectin CL-K1. RT-PCR analyses showed CL-K1 mRNA is present in all organs. The deduced amino acid sequence and the data from immunostaining of CL-K1 cDNA expressing CHO cells revealed that CL-K1 is expressed as a secreted protein. CL-K1 is found in blood by immunoblotting and partial amino acid analyses. CL-K1 showed Ca(2+)-dependent sugar binding activity of fucose and weakly mannose but not N-acetyl-galactosamine, N-acetyl-glucosamine, or maltose, though mannose-binding lectin (MBL) containing similar amino acid motif. CL-K1 can recognize specially several bacterial saccharides due to specific sugar-binding character. Elucidation of the role of two ancestor collectins of CL-K1 and CL-L1 could lead to see the biological function of collectin family.
Authors:
Hiroyuki Keshi; Takashi Sakamoto; Takao Kawai; Katsuki Ohtani; Tsuyoshi Katoh; Seong-Jae Jang; Wataru Motomura; Takayuki Yoshizaki; Mitsuko Fukuda; Satoshi Koyama; Jun Fukuzawa; Atsushi Fukuoh; Itsuro Yoshida; Yasuhiko Suzuki; Nobutaka Wakamiya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology and immunology     Volume:  50     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-12-20     Completed Date:  2007-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1001-13     Citation Subset:  IM    
Affiliation:
Research & Development Center, Fuso Pharmaceutical Industries, Osaka, Osaka, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB119525;  AB119650;  AB119651;  AB119652
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
CHO Cells
Collectins / classification,  genetics*,  isolation & purification*
Cricetinae
Cricetulus
Humans
Kidney / chemistry,  metabolism*
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Collectins; 0/collectin CL-K1, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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