Document Detail


Identification and characterization of a novel human collectin CL-K1.
MedLine Citation:
PMID:  17179669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Collectins are a family of C-type lectins with two characteristic structures, collagen like domains and carbohydrate recognition domains. They recognize carbohydrate antigens on microorganisms and act as host-defense. Here we report the cloning and characterization of a novel collectin CL-K1. RT-PCR analyses showed CL-K1 mRNA is present in all organs. The deduced amino acid sequence and the data from immunostaining of CL-K1 cDNA expressing CHO cells revealed that CL-K1 is expressed as a secreted protein. CL-K1 is found in blood by immunoblotting and partial amino acid analyses. CL-K1 showed Ca(2+)-dependent sugar binding activity of fucose and weakly mannose but not N-acetyl-galactosamine, N-acetyl-glucosamine, or maltose, though mannose-binding lectin (MBL) containing similar amino acid motif. CL-K1 can recognize specially several bacterial saccharides due to specific sugar-binding character. Elucidation of the role of two ancestor collectins of CL-K1 and CL-L1 could lead to see the biological function of collectin family.
Authors:
Hiroyuki Keshi; Takashi Sakamoto; Takao Kawai; Katsuki Ohtani; Tsuyoshi Katoh; Seong-Jae Jang; Wataru Motomura; Takayuki Yoshizaki; Mitsuko Fukuda; Satoshi Koyama; Jun Fukuzawa; Atsushi Fukuoh; Itsuro Yoshida; Yasuhiko Suzuki; Nobutaka Wakamiya
Related Documents :
23879219 - A new pyruvate oxidase biosensor based on 3-mercaptopropionic acid/6-aminocaproic acid ...
24114779 - Very fast electrophoretic determination of creatinine and uric acid in human urine usin...
6336309 - Isolation and nucleotide sequence of a cdna encoding the precursor of mouse nerve growt...
1717589 - Mutational analysis of the cross-reactive idiotype of the a strain mouse.
9339549 - Purification and characterization of ferredoxin-sulfite reductase from turnip (brassica...
21557089 - Degradation and detoxification of acid orange 52 by pseudomonas putida mt-2: a laborato...
16778459 - Acceleration of de novo cholesterol synthesis in the epidermis influences desquamation ...
12787129 - The importance of cationic amino acid transporter expression in human skin.
978439 - Pharmaceutical applications of solid dispersion systems: dissolution of griseofulvin-su...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology and immunology     Volume:  50     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-12-20     Completed Date:  2007-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1001-13     Citation Subset:  IM    
Affiliation:
Research & Development Center, Fuso Pharmaceutical Industries, Osaka, Osaka, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB119525;  AB119650;  AB119651;  AB119652
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
CHO Cells
Collectins / classification,  genetics*,  isolation & purification*
Cricetinae
Cricetulus
Humans
Kidney / chemistry,  metabolism*
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Collectins; 0/collectin CL-K1, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Recombinant vaccinia DIs expressing simian immunodeficiency virus gag and pol in mammalian cells ind...
Next Document:  Pretreatment with N-nitro-L-arginine methyl ester improved oxygenation after inhalation of nitric ox...