Document Detail


Identification and characterization of a novel fibronectin-binding protein gene from Streptococcus equi subspecies zooepidemicus strain VTU211.
MedLine Citation:
PMID:  15949927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work describes the cloning and sequencing of genes encoding fibronectin-binding proteins from Streptococcus equi subspecies zooepidemicus strain VTU211. A gene encoding a cell-wall protein FNZ was amplified and sequenced. In the same bacterial strain, a second gene termed fnz2 was now discovered, encoding another fibronectin-binding protein (FNZ2). The complete amino acid sequence encoded by fnz2 was deduced and compared to that deduced from fnz. The sequence comparison of the fnz and fnz2 predicted that fibronectin-binding activity is localizing a domain in the C terminal part of FNZ2, since this domain is composed of three repeats, which contain a motif similar to what has earlier been found in other fibronectin-binding proteins in streptococci. Three parts of fnz2 [fnz2(1-8), fnz2(2-4), and fnz2(4-3)] were amplified using polymerase chain reaction and ligated into an expression vector, and recombinant FNZ2 proteins were produced in Escherichia coli. Fibronectin bound to the FNZ2(1-8) [amino acids 212-396] and FNZ2(2-4) (amino acids 36-448) but not to the FNZ2(4-3) (amino acids 36-191) in a Western ligand blot, showing that repeat domain of FNZ2 protein was sufficient for binding of fibronectin. Purified FNZ2(2-4) protein was also shown to display collagen-binding activity to collagen-coated microtiter wells. These results show that recombinant FNZ2 has fibronectin- and collagen-binding activities.
Authors:
Kyongsu Hong
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  45     ISSN:  0928-8244     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-29     Completed Date:  2005-10-27     Revised Date:  2010-10-19    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  231-7     Citation Subset:  IM    
Affiliation:
Laboratories for Bioengineering and Research, JCR Pharmaceuticals Co., Ltd., 2-2-10 Murotani, Nishi-ku, Kobe 651-2241, Japan. kou@jcrpharm.co.jp
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MeSH Terms
Descriptor/Qualifier:
Adhesins, Bacterial / chemistry,  genetics*,  metabolism*
Amino Acid Sequence
Animals
Base Sequence
Binding Sites / genetics
Cloning, Molecular
Collagen / metabolism
DNA, Bacterial / genetics
Fibronectins / metabolism*
Genes, Bacterial*
Humans
Molecular Sequence Data
Protein Binding
Protein Structure, Tertiary
Recombinant Proteins / chemistry,  genetics,  metabolism
Streptococcus equi / genetics*,  metabolism*
Chemical
Reg. No./Substance:
0/Adhesins, Bacterial; 0/DNA, Bacterial; 0/Fibronectins; 0/Recombinant Proteins; 0/fibronectin-binding proteins, bacterial; 9007-34-5/Collagen

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