| Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic. | |
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MedLine Citation:
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PMID: 2459116 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rat monoclonal antibody GoH3 identifies a complex of glycoproteins Ic and IIa on human and mouse platelets. The GoH3 epitope is located on glycoprotein Ic. A novel glycoprotein complex is identified by GoH3 on the surface membranes of mouse mammary epithelial tumor cells. This antigen complex is composed of glycoprotein Ic noncovalently associated with a monomor or a disulfide-linked multimer of a high molecular weight glycoprotein (Ic-binding protein (IcBP]. Glycoprotein Ic is synthesized as a large precursor with asparagine N-linked high mannose oligosaccharides. Processing of this precursor involves a proteolytic cleavage of the large polypeptides into two smaller disulfide-linked polypeptide chains, Ic alpha (heavy) and Ic beta (light), and conversion of the majority of the high mannose oligosaccharides into complex-type glycans. Likewise, glycoprotein IcBP is initially glycosylated with high mannose asparagine N-linked oligosaccharides which are processed to complex units in the mature form. Association of glycoprotein Ic with IcBP occurs within the cell soon after their synthesis. The kinetics of labeling show non-coordinate processing consistent with the idea that the concentration of glycoprotein Ic limits complex formation and the subsequent processing of glycoprotein IcBP. |
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Authors:
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A Sonnenberg; F Hogervorst; A Osterop; F E Veltman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 263 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1988 Oct |
Date Detail:
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Created Date: 1988-11-07 Completed Date: 1988-11-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 14030-8 Citation Subset: IM |
Affiliation:
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Division of Tumor Biology, Netherlands Cancer Institute (Antoni van Leeuwenhoekhuis), Amsterdam. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal* Antigen-Antibody Complex / analysis* Antigens, Neoplasm / immunology* Antigens, Surface / immunology* Cell Line Epitopes / analysis Humans Kinetics Laminin / biosynthesis Macromolecular Substances Mammary Neoplasms, Experimental / immunology*, metabolism Mice Platelet Membrane Glycoproteins / immunology* Tunicamycin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigen-Antibody Complex; 0/Antigens, Neoplasm; 0/Antigens, Surface; 0/Epitopes; 0/Laminin; 0/Macromolecular Substances; 0/Platelet Membrane Glycoproteins; 11089-65-9/Tunicamycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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