Document Detail


Identification and characterization of neural crest-derived cells in adult periodontal ligament of mice.
MedLine Citation:
PMID:  22704955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Cells derived from the neural crest (NC) contribute to the development of several adult tissues, including tooth and periodontal tissue. Here, two transgenic lines, Wnt1-Cre/ZEG and P0-Cre/ZEG, were analysed to determine the fate and distribution of neural crest cells (NCCs) in adult mouse periodontal ligament (PDL).
DESIGN: Paraffin-embedded and decalcified histology samples were prepared from Wnt1-Cre/ZEG and P0-Cre/ZEG mice that were 4-, 8-, or 12-weeks old. Expression of GFP (NC-derived cells), NC-markers (Slug, AP-2 alpha, HNK-1, p75NTR and Nestin), and mesenchymal stem cell markers (CD29 and STRO-1) were examined using immunohistochemistry.
RESULTS: In four-week-old Wnt1-Cre/ZEG mice, GFP((+)) NC-derived cells were specifically detected in the mid-zone of PDL. The GFP((+)) cells constituted 1.4% of all cells in PDL, and this percentage decreased as the mice aged. The distribution and prevalence of GFP((+)) cells were comparable between Wnt1-Cre/ZEG and P0-Cre/ZEG mice. NC-marker((+)) cells were expressed only in GFP((+)) cells while MSC markers were detected only in GFP((-)) cells.
CONCLUSION: The prevalence and specific distribution of NC-derived cells in adult PDL of Wnt1-Cre/ZEG and P0-Cre/ZEG mouse were examined. Interestingly, various NC markers, including markers for undifferentiated NCCs, were still expressed at high levels in GFP((+)) cells. These observations may indicate that labelled cells in the Wnt1-Cre/ZEG and P0-Cre/ZEG mice did not constituted all NC-derived cells, but rather an interesting subset of NC-derived cells. These findings may be useful in understanding the homeostatic character of the PDL and contribute to establishing successful periodontal tissue maintenance.
Authors:
Masaru Kaku; Yoshihiro Komatsu; Yoshiyuki Mochida; Mitsuo Yamauchi; Yuji Mishina; Ching-Chang Ko
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2012-06-15
Journal Detail:
Title:  Archives of oral biology     Volume:  57     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-26     Completed Date:  2013-05-24     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1668-75     Citation Subset:  D; IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Separation / methods
Immunohistochemistry
Mice
Mice, Transgenic
Multipotent Stem Cells / cytology*
Neural Crest / cytology*
Periodontal Ligament / cytology*
Grant Support
ID/Acronym/Agency:
DE019527/DE/NIDCR NIH HHS; ES071003-11/ES/NIEHS NIH HHS; K08DE018695/DE/NIDCR NIH HHS; K99DE021054/DE/NIDCR NIH HHS; R01 DE019527/DE/NIDCR NIH HHS; R01 DE020843/DE/NIDCR NIH HHS; R01DE020843/DE/NIDCR NIH HHS; R21 AR057451/AR/NIAMS NIH HHS
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