| Identification and characterization of a major liver lysophosphatidylcholine acyltransferase. | |
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MedLine Citation:
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PMID: 18195019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphatidylcholine (PC) is synthesized through the Kennedy pathway, but more than 50% of PC is remodeled through the Lands cycle, i.e. the deacylation and reacylation of PC to attain the final and proper fatty acids within PC. The reacylation step is catalyzed by lysophosphatidylcholine acyltransferase (LPCAT), and we report here the identification of a novel LPCAT, which we named LPCAT3. LPCAT3 belongs to the membrane-bound O-acyltransferase (MBOAT) family and encodes a protein of 487 amino acids with a calculated molecular mass of 56 kDa. Membranes from HEK293 cells overexpressing LPCAT3 showed significantly increased LPCAT activity as assessed by thin layer chromatography analysis with substrate preference toward unsaturated fatty acids. LPCAT3 is localized within the endoplasmic reticulum and is primarily expressed in metabolic tissues including liver, adipose, and pancreas. In a human hepatoma Huh7 cells, RNA interference-mediated knockdown of LPCAT3 resulted in virtually complete loss of membrane LPCAT activity, suggesting that LPCAT3 is primarily responsible for hepatic LPCAT activity. Furthermore, peroxisome proliferator-activated receptor alpha agonists dose-dependently regulated LPCAT3 in liver in a peroxisome proliferator-activated receptor alpha-dependent fashion, implicating a role of LPCAT3 in lipid homeostasis. Our studies identify a long-sought enzyme that plays a critical role in PC remodeling in metabolic tissues and provide an invaluable tool for future investigations on how PC remodeling may potentially impact glucose and lipid homeostasis. |
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Authors:
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Yang Zhao; Yan-Qun Chen; Tabetha M Bonacci; David S Bredt; Shuyu Li; William R Bensch; David E Moller; Mark Kowala; Robert J Konrad; Guoqing Cao |
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Publication Detail:
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Type: Journal Article Date: 2008-01-14 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 283 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-24 Completed Date: 2008-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 8258-65 Citation Subset: IM |
Affiliation:
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Lilly Research Laboratories, Eli Lilly & Company, Indianapolis, IN 46285, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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RefSeq/NM_005768 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Acylglycerophosphocholine O-Acyltransferase
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chemistry,
genetics,
metabolism* Amino Acid Sequence Animals Cell Line Cercopithecus aethiops Conserved Sequence Gene Expression Regulation, Enzymologic / drug effects Humans Kinetics Liver / enzymology* Molecular Sequence Data Organ Specificity PPAR alpha / agonists, metabolism RNA, Small Interfering / genetics, metabolism Sequence Alignment Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/PPAR alpha; 0/RNA, Small Interfering; EC 2.3.1.23/1-Acylglycerophosphocholine O-Acyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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