Document Detail


Identification and characterization of differentiation-dependent Schwann cell surface antigens by novel monoclonal antibodies: introduction of a marker common to the non-myelin-forming phenotype.
MedLine Citation:
PMID:  9063728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In an attempt to identify and characterize novel Schwann cell surface molecules with putative functions during development, maintenance, and regeneration of the peripheral nervous system (PNS), we have produced monoclonal antibodies against viable neonatal rat Schwann cells. Using a sensitive live cell ELISA protocol, three monoclonal antibodies reactive with cultured Schwann cells, designated 27B10, 26F2, and 27C7 were isolated. The 27B10 and 26F2 antibodies specifically labelled forskolin-stimulated secondary Schwann cells in vitro as determined by live cell ELISA implying that the expression of the antigens in situ is regulated by axonal contact. The observation that the antigens seemed to be associated with both Schwann cell phenotypes clearly discriminated them from the well characterized myelin proteins as well as from molecules known to be confined to the non-myelin-forming phenotype. Interestingly, both antigens were found to be concentrated at the nodes of Ranvier. Further studies therefore have to show whether the identified antigens share structural or functional homology with adhesion or channel molecules, which display a similar distribution. Following transection of the adult sciatic nerve, the 26F2 antigen was rapidly down-regulated in the distal nerve stump. The 27C7 antibody reacted with an 80 kDa cell surface molecule common to non-myelin-forming Schwann cells. No differences in expression of the antigen between forskolin-treated and untreated Schwann cells in vitro were found, suggesting that the antigen is expressed independently from axonal contact. Two weeks after nerve transection in the absence of myelinating Schwann cells, the antigen was associated with S-100-positive Schwann cells of the distal nerve stump. The antigen was found to be expressed also by non-neuronal tissues, the level of the protein declined towards the adult stage. Comparison of the 27C7 antigen with previously described marker molecules suggests that we have identified a novel Schwann cell surface antigen of the non-myelin-forming phenotype.
Authors:
K Wewetzer; C Grothe; B Christ; B Seilheimer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Glia     Volume:  19     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-24     Completed Date:  1997-06-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  213-26     Citation Subset:  IM    
Affiliation:
University of Freiburg, Institute of Anatomy II, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antibodies, Monoclonal / immunology
Antigens, Surface / immunology*
Biological Markers*
Blotting, Western
Cell Differentiation*
Enzyme-Linked Immunosorbent Assay
Female
Ganglia, Spinal
Genotype
Mice
Mice, Inbred BALB C
Myelin Sheath
Phenotype
Rats
Schwann Cells / immunology*
Sciatic Nerve
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Surface; 0/Biological Markers

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