Document Detail


Identification and characterization of differentially expressed genes involved in pharmacological activities of roots of Panax notoginseng during plant growth.
MedLine Citation:
PMID:  18283465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Panax notoginseng is a highly valued Chinese medicinal herb. To understand the molecular mechanism of the much higher pharmacological activities of roots of 3-year-old plants over 1-year-old ones,two cDNA libraries were constructed using the suppression subtractive hybridization (SSH) method. Positive cDNA clones from each of the two libraries were randomly selected for dot-blotting analysis. A total of 110 genes were highly expressed in 3-year-old roots and 80 genes in 1-year-old roots. Of these, 87 cDNA fragments were sequenced, assembled, and compared with sequences in GenBank, and 81 individual cDNAs were identified. These cDNAs were the first expressed sequence tags of P. notoginseng in GenBank. The result of reverse transcription PCR analysis of six genes was consistent with that of the dot-blot analysis. The global gene expression profile showed that there were significant differences between 1- and 3-year-old roots of P. notoginseng plants. Some important structural and regulatory genes which may be involved in isoprenoid biosynthesis were found to be over-expressed in 3-year-old roots, such as genes encoding 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphat-synthase (IspG-protein), multi-copper oxidase type I family protein, NADH flavin oxidoreductase, lipase and aconitase.
Authors:
Fengmei He; Yongping Zhu; Yizheng Zhang
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Publication Detail:
Type:  Journal Article     Date:  2008-02-19
Journal Detail:
Title:  Plant cell reports     Volume:  27     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-11     Completed Date:  2008-09-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  923-30     Citation Subset:  IM    
Affiliation:
Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, People's Republic of China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EC599966;  EC599967;  EC599968;  EC599969;  EC599970;  EC599971;  EC599972;  EC599973;  EC599974;  EC599975;  EC599976;  EC599977;  EC599978;  EC599979;  EC599980;  EC599981;  EC599982;  EC599983;  EC599984;  EC599985;  EC599986;  EC599987;  EC599988;  EC599989;  EC599990;  EC599991;  EC599992;  EC599993;  EC599994;  EC599995;  EC599996;  EC599997;  EC599998;  EC599999;  EC600000;  EC600001;  EC600002;  EC600003;  EC600004;  EC600005;  EC600006;  EC600007;  EC600008;  EC600009;  EC600010;  EC600011;  EC600012;  EC600013;  EC600014;  EC600015;  EC600016;  EC600017;  EC600018;  EC600019;  EC600020;  EC600021;  EC600022;  EC600023;  EC600024;  EC600025;  EC600026;  EC600027;  EC600028;  EC600029;  EC600030;  EC600031;  EC600032;  EC600033;  EC600034;  EC600035;  EC600036;  EC600037;  EC600038;  EC600039;  EC600040;  EC600041;  EC600042;  EC600043;  EC600044;  EC600045;  EC600046;  EC600047;  EC600048;  EC600049;  EC997073;  EC997074;  EC997075;  EC997076;  EC997077;  EC997078;  EC997079;  EC997080;  EC997081
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MeSH Terms
Descriptor/Qualifier:
Expressed Sequence Tags
Gene Expression Profiling*
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene Library
Molecular Sequence Data
Panax notoginseng / genetics*,  growth & development
Plant Roots / genetics*,  growth & development
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA

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