| Identification and characterization of differentially expressed genes involved in pharmacological activities of roots of Panax notoginseng during plant growth. | |
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MedLine Citation:
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PMID: 18283465 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Panax notoginseng is a highly valued Chinese medicinal herb. To understand the molecular mechanism of the much higher pharmacological activities of roots of 3-year-old plants over 1-year-old ones,two cDNA libraries were constructed using the suppression subtractive hybridization (SSH) method. Positive cDNA clones from each of the two libraries were randomly selected for dot-blotting analysis. A total of 110 genes were highly expressed in 3-year-old roots and 80 genes in 1-year-old roots. Of these, 87 cDNA fragments were sequenced, assembled, and compared with sequences in GenBank, and 81 individual cDNAs were identified. These cDNAs were the first expressed sequence tags of P. notoginseng in GenBank. The result of reverse transcription PCR analysis of six genes was consistent with that of the dot-blot analysis. The global gene expression profile showed that there were significant differences between 1- and 3-year-old roots of P. notoginseng plants. Some important structural and regulatory genes which may be involved in isoprenoid biosynthesis were found to be over-expressed in 3-year-old roots, such as genes encoding 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphat-synthase (IspG-protein), multi-copper oxidase type I family protein, NADH flavin oxidoreductase, lipase and aconitase. |
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Authors:
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Fengmei He; Yongping Zhu; Yizheng Zhang |
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Publication Detail:
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Type: Journal Article Date: 2008-02-19 |
Journal Detail:
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Title: Plant cell reports Volume: 27 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-11 Completed Date: 2008-09-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 923-30 Citation Subset: IM |
Affiliation:
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Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, People's Republic of China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EC599966; EC599967; EC599968; EC599969; EC599970; EC599971; EC599972; EC599973; EC599974; EC599975; EC599976; EC599977; EC599978; EC599979; EC599980; EC599981; EC599982; EC599983; EC599984; EC599985; EC599986; EC599987; EC599988; EC599989; EC599990; EC599991; EC599992; EC599993; EC599994; EC599995; EC599996; EC599997; EC599998; EC599999; EC600000; EC600001; EC600002; EC600003; EC600004; EC600005; EC600006; EC600007; EC600008; EC600009; EC600010; EC600011; EC600012; EC600013; EC600014; EC600015; EC600016; EC600017; EC600018; EC600019; EC600020; EC600021; EC600022; EC600023; EC600024; EC600025; EC600026; EC600027; EC600028; EC600029; EC600030; EC600031; EC600032; EC600033; EC600034; EC600035; EC600036; EC600037; EC600038; EC600039; EC600040; EC600041; EC600042; EC600043; EC600044; EC600045; EC600046; EC600047; EC600048; EC600049; EC997073; EC997074; EC997075; EC997076; EC997077; EC997078; EC997079; EC997080; EC997081 |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Expressed Sequence Tags Gene Expression Profiling* Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gene Library Molecular Sequence Data Panax notoginseng / genetics*, growth & development Plant Roots / genetics*, growth & development Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA |
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